Diffusion of CO2and other gases inside leaves

Abstract

SUMMARY: Diffusion of CO 2 in the intercellular airspaces of the leaf mesophyll is one of the many processes that can limit photosynthetic carbon assimilation there. This limitation has been largely neglected in recent years, but both theoretical and empirical evidence is presented showing that it can be substantial, reducing CO 2 assimilation rates by 25% or more in some leaves. Intercellular diffusion is fundamentally a three‐dimensional process, because CO 2 enters the leaf through discrete stomata, and not through a uniformly porous epidermis. Modelling it in one dimension can cause major underestimation of its limiting effects. Resistance and conductance models often fail to account well for the limitation, in part because they are usually one‐dimensional representations, but also because they treat continuously interacting processes as if they were sequential. A three‐dimensional diffusion model is used to estimate the intercellular limitation in leaves of different structural types. Intercellular gaseous diffusion probably limits CO 2 assimilation by at most a few percent in many of the agricultural plants commonly studied by laboratory physiologists, as they usually have thin, amphistaomatous leaves. However, it may cause substantial limitations in the thicker, often hypostomatous leaves of the wild plants that occupy large areas of the earth. Several physiological implications of intercellular diffusion are discussed. I close by reiterating published suggestions that the gas‐exchange parameter commonly termed p i should be renamed p es(es for evaporating surfaces) to avoid the confusing implication that p 1, is‘the’intercellular CO 2 pressure. ContentsSummary449I.Introduction450II.Theory of intercellular gaseous diffusion451III.Evidence regarding intercellular gaseous diffusion hmitations461IV.Discussion of assumptions made in §11.1470V.Physiological implications of intercellular gaseous diffusion471VI.Conclusions475Acknowledgements476Appendix, Example showing breakdown of resistance analogy for diffusion with distributed uptake476References477