The evolution of RNA editing and pentatricopeptide repeat genes

Abstract

Summary: The pentatricopeptide repeat (PPR) is a degenerate 35‐amino‐acid structural motif identified from analysis of the sequenced genome of the model plant Arabidopsis thaliana. From the wealth of sequence information now available from plant genomes, the PPR protein family is now known to be one of the largest families in angiosperm species, as most genomes encode 400–600 members. As the number of PPR genes is generally only c. 10–20 in other eukaryotic organisms, including green algae, the family has obviously greatly expanded during land plant evolution. This provides a rare opportunity to study selection pressures driving a 50‐fold expansion of a single gene family. PPR proteins are sequence‐specific RNA‐binding proteins involved in many aspects of RNA processing in organelles. In this review, we will summarize our current knowledge about the evolution of PPR genes, and will discuss the relevance of the dramatic expansion in the family to the functional diversification of plant organelles, focusing primarily on RNA editing. Contents Summary 37 I. Introduction 37 II. Expansion of the PPR family 38 III. RNA editing and the evolution of PPR genes 40 IV. Sequence drift in the organelle genomes of early land plants 41 V. Loss of RNA editing in angiosperms 43 VI. Other functions besides editing 44 VII. Conclusion 44 References 44