The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Bovine Brain Endothelial Cells Express Tight Junctions and Monoamine Oxidase Activity in Long‐Term Culture
Abstract
Abstract: The passage of substances across the blood‐brain barrier is regulated by cerebral capillaries which possess certain distinctly different morphological and enzymatic properties compared to capillaries of other organs. Investigations of the functional characteristics of brain capillaries have been facilitated by the use of cultured brain endothelial cells, but in most studies a number of characteristics of the in vivo system are lost. To provide an in vitro system for studies of brain capillary functions, we developed a method of isolating and producing a large number of bovine brain capillary endothelial cells. These cells, absolutely free of pericyte contamination, are subcultured, at the split ratio of 1:20 (20‐fold increase of the cultured surface), with no apparent changes in cell morphology up to the fiftieth generation (10 passages). Retention of endothelial‐specific characteristics (factor V11I‐related antigen, angiotensin‐converting enzyme, and nonthrombogenic surface) is shown for brain capillary‐derived endothelial cells up to passage 10, even after frozen storage at passage 3. Furthermore, we showed that bovine brain capillary endothelial cells retain, up to the fiftieth generation, some of the characteristics of the blood‐brain barrier: occurrence of tight junctions, paucity of pinocytotic vesicles, and monoamine oxidase activity
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Citations
Cites
FINE STRUCTURAL LOCALIZATION OF A BLOOD-BRAIN BARRIER TO EXOGENOUS PEROXIDASE
Reese, T. S., & Karnovsky, M. J. (1967) · The Journal of Cell Biology · doi:10.1083/jcb.34.1.207 · Cited by 2,629
Cited by
Helms, H. C., Abbott, N. J., Burek, M., Cecchelli, R., Couraud, P.-O., Deli, M. A., Förster, C., Galla, H. J., Romero, I. A., Shusta, E. V., et al. (2016) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1177/0271678x16630991 · Cited by 843
Self-citationMitochondria and Endothelial Function
Kluge, M. A., Fetterman, J. L., & Vita, J. A. (2013) · Circulation Research · doi:10.1161/circresaha.111.300233 · Cited by 508
A New Function for the LDL Receptor: Transcytosis of LDL across the Blood–Brain Barrier
Dehouck, B., Fenart, L., Dehouck, M.-P., Pierce, A., Torpier, G., & Cecchelli, R. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.138.4.877 · Cited by 490
Self-citationKreuter, J., Ramge, P., Petrov, V., Hamm, S., Gelperina, S. E., Engelhardt, B., Alyautdin, R., von Briesen, H., & Begley, D. J. (2003) · Pharmaceutical Research · doi:10.1023/a:1022604120952 · Cited by 469
In vitro model for evaluating drug transport across the blood–brain barrier
Cecchelli, R., Dehouck, B., Descamps, L., Fenart, L., Buée-Scherrer, V, Duhem, C., Lundquist, S., Rentfel, M., Torpier, G., & Dehouck, M. P. (1999) · Advanced Drug Delivery Reviews · doi:10.1016/s0169-409x(98)00083-0 · Cited by 383
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Metadata
- Authors
- Méresse, S., Dehouck, M., Delorme, P., Bensaïd, M., Tauber, J., Delbart, C., Fruchart, J., & Cecchelli, R.
- Venue
- Journal of Neurochemistry
- Publisher
- Wiley
- Published
- 1989-11
- Volume
- 53
- Issue
- 5
- Pages
- 1363-1371
- ISSN
- 0022-3042 (ISSN-L)
- 1471-4159
- OpenAlex
- W2117697959
- Cited by
- 204
- References
- 1
- FWCI
- 5.72
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Bovine Brain Endothelial Cells Express Tight Junctions and Monoamine Oxidase Activity in Long‐Term Culture. https://app.cochat.ai/papers/10.1111/j.1471-4159.1989.tb08526.x
- The paper
- Méresse, S., Dehouck, M., Delorme, P., Bensaïd, M., Tauber, J., Delbart, C., Fruchart, J., & Cecchelli, R. (1989). Bovine Brain Endothelial Cells Express Tight Junctions and Monoamine Oxidase Activity in Long‐Term Culture. Journal of Neurochemistry. https://doi.org/10.1111/j.1471-4159.1989.tb08526.x
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
