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Universal primer cocktails for fish DNA barcoding
Abstract
Reliable recovery of the 5′ region of the cytochrome c oxidase 1 (COI) gene is critical for the ongoing effort to gather DNA barcodes for all fish species. In this study, we develop and test primer cocktails with a view towards increasing the efficiency of barcode recovery. Specifically, we evaluate the success of polymerase chain reaction amplification and the quality of resultant sequences using three primer cocktails on DNA extracts from representatives of 94 fish families. Our results show that M13‐tailed primer cocktails are more effective than conventional degenerate primers, allowing barcode work on taxonomically diverse samples to be carried out in a high‐throughput fashion.
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Cited by
Species identification in cell culture: a two-pronged molecular approach
Cooper, J. K., Sykes, G., King, S., Cottrill, K., Ivanova, N. V., Hanner, R., & Ikonomi, P. (2007) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-007-9060-2 · Cited by 174
Self-citationMolecular diversity of Uzbekistan’s fishes assessed with DNA barcoding
Sheraliev, B., & Peng, Z. (2021) · Scientific Reports · doi:10.1038/s41598-021-96487-1 · Cited by 42
Metadata
- Authors
- IVANOVA, N. V., ZEMLAK, T. S., HANNER, R. H., & HEBERT, P. D. N.
- Venue
- Molecular Ecology Notes
- Publisher
- Wiley
- Published
- 2007-03-19
- Volume
- 7
- Issue
- 4
- Pages
- 544-548
- ISSN
- 1471-8278 (ISSN-L)
- 1471-8286
- OpenAlex
- W2103330559
- Cited by
- 1,488
- References
- 0
- FWCI
- 21.89
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Universal primer cocktails for fish DNA barcoding. https://app.cochat.ai/papers/10.1111/j.1471-8286.2007.01748.x
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- IVANOVA, N. V., ZEMLAK, T. S., HANNER, R. H., & HEBERT, P. D. N. (2007). Universal primer cocktails for fish DNA barcoding. Molecular Ecology Notes. https://doi.org/10.1111/j.1471-8286.2007.01748.x
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