The DOI matches its Crossref record; 58 of the 58 references it cites were found in a registry or an index.
Cellular senescence in human myoblasts is overcome by human telomerase reverse transcriptase and cyclin‐dependent kinase 4: consequences in aging muscle and therapeutic strategies for muscular dystrophies
Open accessAbstract
Summary: Cultured human myoblasts fail to immortalize following the introduction of telomerase. The availability of an immortalization protocol for normal human myoblasts would allow one to isolate cellular models from various neuromuscular diseases, thus opening the possibility to develop and test novel therapeutic strategies. The parameters limiting the efficacy of myoblast transfer therapy (MTT) could be assessed in such models. Finally, the presence of an unlimited number of cell divisions, and thus the ability to clone cells after experimental manipulations, reduces the risks of insertional mutagenesis by many orders of magnitude. This opportunity for genetic modification provides an approach for creating a universal donor that has been altered to be more therapeutically useful than its normal counterpart. It can be engineered to function under conditions of chronic damage (which are very different than the massive regeneration conditions that recapitulate normal development), and to overcome the biological problems such as cell death and failure to proliferate and migrate that limit current MTT strategies. We describe here the production and characterization of a human myogenic cell line, LHCN‐M2, that has overcome replicative aging due to the expression of telomerase and cyclin‐dependent kinase 4. We demonstrate that it functions as well as young myoblasts in xenotransplant experiments in immunocompromized mice under conditions of regeneration following muscle damage.
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Cites
Extension of Life-Span by Introduction of Telomerase into Normal Human Cells
Bodnar, A. G., Ouellette, M., Frolkis, M., Holt, S. E., Chiu, C.-P., Morin, G. B., Harley, C. B., Shay, J. W., Lichtsteiner, S., & Wright, W. E. (1998) · Science · doi:10.1126/science.279.5349.349 · Cited by 4,975
Self-citationCollins, C. A., Olsen, I., Zammit, P. S., Heslop, L., Petrie, A., Partridge, T. A., & Morgan, J. E. (2005) · Cell · doi:10.1016/j.cell.2005.05.010 · Cited by 1,445
Vaziri, H., & Benchimol, S. (1998) · Current Biology · doi:10.1016/s0960-9822(98)70109-5 · Cited by 1,003 · cited 1×
“The introduction of the cDNA coding for hTERT leads to telomere elongation and extended lifespan in a number of cell types including fibroblasts, retinal pigment cells and endothelial cells (Bodnar et al ., 1998; Vaziri & Benchinol, 1998 ).”
BackgroundConversion of mdx myofibres from dystrophin-negative to -positive by injection of normal myoblasts
Partridge, T. A., Morgan, J. E., Coulton, G. R., Hoffman, E. P., & Kunkel, L. M. (1989) · Nature · doi:10.1038/337176a0 · Cited by 878
Mesoangioblast stem cells ameliorate muscle function in dystrophic dogs
Sampaolesi, M., Blot, S., D’Antona, G., Granger, N., Tonlorenzi, R., Innocenzi, A., Mognol, P., Thibaud, J.-L., Galvez, B. G., Barthélémy, I., et al. (2006) · Nature · doi:10.1038/nature05282 · Cited by 769
Sherr, C. J., & DePinho, R. A. (2000) · Cell · doi:10.1016/s0092-8674(00)00046-5 · Cited by 745
Chimeric nucleases stimulate gene targeting in human cells
Porteus, M. H., & Baltimore, D. (2003) · Science · doi:10.1126/science.1078395 · Cited by 741
Barton-Davis, E. R., Shoturma, D. I., Musaro, A., Rosenthal, N., & Sweeney, H. L. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.26.15603 · Cited by 700
Sampaolesi, M., Torrente, Y., Innocenzi, A., Tonlorenzi, R., D'Antona, G., Pellegrino, M. A., Barresi, R., Bresolin, N., De Angelis, M. G. C., Campbell, K. P., et al. (2003) · Science · doi:10.1126/science.1082254 · Cited by 641
Immortalization of human bronchial epithelial cells in the absence of viral oncoproteins
Ramirez, R. D., Sheridan, S., Girard, L., Sato, M., Kim, Y., Pollack, J., Peyton, M., Zou, Y., Kurie, J. M., DiMaio, J. M., et al. (2004) · Cancer Research · doi:10.1158/0008-5472.can-04-3703 · Cited by 607
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Cited by
Engineered In Vitro Disease Models
Benam, K. H., Dauth, S., Hassell, B., Herland, A., Jain, A., Jang, K.-J., Karalis, K., Kim, H. J., MacQueen, L., Mahmoodian, R., et al. (2015) · Annual Review of Pathology: Mechanisms of Disease · doi:10.1146/annurev-pathol-012414-040418 · Cited by 554
DUX4 recruits p300/CBP through its C-terminus and induces global H3K27 acetylation changes
Choi, S. H., Gearhart, M. D., Cui, Z., Bosnakovski, D., Kim, M., Schennum, N., & Kyba, M. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw141 · Cited by 196
Functional dysregulation of stem cells during aging: a focus on skeletal muscle stem cells
García‐Prat, L., Sousa‐Victor, P., & Muñoz‐Cánoves, P. (2013) · The FEBS Journal · doi:10.1111/febs.12221 · Cited by 152
Gut microbiome catabolites as novel modulators of muscle cell glucose metabolism
Houghton, M. J., Kerimi, A., Mouly, V., Tumova, S., & Williamson, G. (2019) · The FASEB Journal · doi:10.1096/fj.201801209r · Cited by 92
Self-citationRibas, F., Villarroya, J., Hondares, E., Giralt, M., & Villarroya, F. (2014) · Biochemical Journal · doi:10.1042/bj20140403 · Cited by 69
Metadata
- Authors
- Zhu, C., Mouly, V., Cooper, R. N., Mamchaoui, K., Bigot, A., Shay, J. W., Di Santo, J. P., Butler‐Browne, G. S., & Wright, W. E.
- Venue
- Aging Cell
- Publisher
- Wiley
- Published
- 2007-05-08
- Volume
- 6
- Issue
- 4
- Pages
- 515-523
- ISSN
- 1474-9718 (ISSN-L)
- 1474-9726
- OpenAlex
- W2140692553
- Cited by
- 299
- References
- 58
- FWCI
- 6.53
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Zhu, C., Mouly, V., Cooper, R. N., Mamchaoui, K., Bigot, A., Shay, J. W., Di Santo, J. P., Butler‐Browne, G. S., & Wright, W. E. (2007). Cellular senescence in human myoblasts is overcome by human telomerase reverse transcriptase and cyclin‐dependent kinase 4: consequences in aging muscle and therapeutic strategies for muscular dystrophies. Aging Cell. https://doi.org/10.1111/j.1474-9726.2007.00306.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
