Architectural (Type IA) Focal Cortical Dysplasia and Parvalbumin Immunostaining in Temporal Lobe Epilepsy
Open accessAbstract
Summary: Purpose:We analyzed 26 surgically treated patients operated on for intractable epilepsy associated with type IA (architectural) cortical dysplasia, to investigate neuropathologic and immunocytochemical features, particularly of the γ‐aminobutyric acid (GABA)ergic system, and to compare the findings with those observed in normal cortex. Methods:. Routinely stained slides and serial sections immunostained for neurofilaments (SMI 311), microtubule‐associated protein‐2 (MAP‐2), neuron‐specific nuclear protein (NeuN), glial fibrillary acidic protein (GFAP), parvalbumin (PV), calbindin (CB), and calretinin (CR) were processed. Some sections were processed by using single‐immunoperoxidase procedures; others were processed for double immunofluorescence labelling and observed by confocal microscopy. The density of inhibitory PV‐immunoreactive interneurons was quantitatively assessed in all patients and control cases by using a two‐dimensional cell‐counting technique on PV immunostained sections. Results:The density of PV‐immunoreactive interneurons was significantly reduced in this group of patients, whereas CB‐ and CR‐ positivity appeared similar to those in normal cortex. In five cases, architectural abnormalities, in addition to those that defined type 1A dysplasia, were present and characterized by abnormal clusters of neurons and laminar cellular loss in superficial cortical laminate. Conclusions:The reduction of PV expression in type IA cortical dysplasia suggests an impairment of the GABAergic system as a possible mechanism for the epileptogenicity; in addition, PV immunoreactivity can be helpful in the neuropathologic characterization of this form of cortical dysplasia.
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Epilepsy and prion disease are among other disorders associated with alterations in PV-INs.
“Epilepsy [63,64] and prion disease [65] are among other disorders that are associated with alterations in PV-INs.”
From Parvalbumin interneuron vulnerability and brain disorders (2021) · cites this paper as [64]
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Terminology and classification of the cortical dysplasias
Palmini, A., Najm, I., AVANZINI, G., Babb, T. L., Guerrini, R., Foldvary‐Schaefer, N., Jackson, G. D., Lüders, H. O., Prayson, R. A., Spreafico, R., et al. (2004) · Neurology · doi:10.1212/01.wnl.0000114507.30388.7e · Cited by 948 · see citing works
Self-citationFocal cortical dysplasia: neuropathological subtypes, EEG, neuroimaging and surgical outcome
Tassi, L. (2002) · Brain · doi:10.1093/brain/awf175 · Cited by 669 · see citing works
Self-citationClassification system for malformations of cortical development
Barkovich, A. J., Kuzniecky, R., Jackson, G. D., Guerrini, R., & Dobyns, W. B. (2001) · Neurology · doi:10.1212/wnl.57.12.2168 · Cited by 526 · see citing works
Ammon's Horn Sclerosis: A Maldevelopmental Disorder Associated with Temporal Lobe Epilepsy
Blümcke, I., Thom, M., & Wiestler, O. D. (2002) · Brain Pathology · doi:10.1111/j.1750-3639.2002.tb00436.x · Cited by 375 · see citing works
Calcium buffering properties of calbindin D28k and parvalbumin in rat sensory neurones.
Chard, P. S., Bleakman, D., Christakos, S., Fullmer, C. S., & Miller, R. J. (1993) · The Journal of Physiology · doi:10.1113/jphysiol.1993.sp019950 · Cited by 346 · see citing works
Visualization of chandelier cell axons by parvalbumin immunoreactivity in monkey cerebral cortex.
DeFelipe, J., Hendry, S. H. C., & Jones, E. G. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.6.2093 · Cited by 338 · see citing works
Excitation and Inhibition in Epilepsy
Engel, J. (1996) · Canadian Journal of Neurological Sciences / Journal Canadien des Sciences Neurologiques · doi:10.1017/s0317167100038464 · Cited by 189 · see citing works
Dysfunction of Synaptic Inhibition in Epilepsy Associated with Focal Cortical Dysplasia
Calcagnotto, M. E., Paredes, M. F., Tihan, T., Barbaro, N. M., & Baraban, S. C. (2005) · Journal of Neuroscience · doi:10.1523/jneurosci.2687-05.2005 · Cited by 182 · see citing works
Pauls, T. L., Cox, J. A., & Berchtold, M. W. (1996) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/0167-4781(95)00221-9 · Cited by 139 · see citing works
Inhibitory Circuits in Human Dysplastic Tissue
Spreafico, R., Tassi, L., Colombo, N., Bramerio, M., GALLI, C., Garbelli, R., Ferrario, A., Russo, G. L., & Munari, C. (2000) · Epilepsia · doi:10.1111/j.1528-1157.2000.tb01576.x · Cited by 110 · see citing works
Self-citationTaylor's Cortical Dysplasia: A Confocal and Ultrastructural Immunohistochemical Study
Garbelli, R., Munari, C., Biasi, S. D., Vitellaro‐Zuccarello, L., Galli, C., Bramerio, M., Mai, R., Battaglia, G. S., & Spreafico, R. (1999) · Brain Pathology · doi:10.1111/j.1750-3639.1999.tb00534.x · Cited by 110 · see citing works
Self-citationRoper, S. N., Eisenschenk, S. J., & King, M. A. (1999) · Epilepsy Research · doi:10.1016/s0920-1211(99)00035-2 · Cited by 93 · see citing works
Microanatomy of the dysplastic neocortex from epileptic patients
Alonso‐Nanclares, L. (2004) · Brain · doi:10.1093/brain/awh331 · Cited by 87 · see citing works
Thom, M., Harding, B. N., Lin, W.-R., Martinian, L., Cross, J. H., & Sisodiya, S. M. (2003) · Acta Neuropathologica · doi:10.1007/s00401-003-0673-6 · Cited by 54 · see citing works
Thom, M., Holton, J. L., D’Arrigo, C., Griffin, B., Beckett, A. A. J., Sisodiya, S. M., Alexiou, D., & Sander, J. W. A. S. (2000) · Neuropathology and Applied Neurobiology · doi:10.1046/j.1365-2990.2000.00229.x · Cited by 46 · see citing works
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Parvalbumin interneuron vulnerability and brain disorders
Ruden, J. B., Dugan, L. L., & Konradi, C. (2021) · Neuropsychopharmacology · doi:10.1038/s41386-020-0778-9 · Cited by 194 · see citing works
- Authors
- Garbelli, R., Meroni, A., Magnaghi, G., Beolchi, M. S., Ferrario, A., Tassi, L., Bramerio, M., & Spreafico, R.
- Affiliations
- Fondazione IRCCS Istituto Neurologico Carlo Besta (IT)
- Azienda Socio Sanitaria Territoriale Grande Ospedale Metropolitano Niguarda (IT)
- Venue
- Epilepsia
- Publisher
- Wiley
- Published
- 2006-06
- Volume
- 47
- Issue
- 6
- Pages
- 1074-1078
- ISSN
- 0013-9580 (ISSN-L)
- 1528-1167
- OpenAlex
- W2069899587
- Cited by
- 47
- References
- 15
- Citations by year
- 2026: 1 · 2024: 1 · 2022: 1 · 2021: 2 · 2020: 2 · 2019: 1 · 2017: 2 · 2016: 1 · 2015: 4 · 2014: 2 · 2013: 4 · 2012: 4
- FWCI
- 2.54
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Epilepsia (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministero della Salute (RF2003)
- Ministero dell’Istruzione, dell’Università e della Ricerca (2003068749)
- Topics
- Health Sciences › Medicine › Psychiatry and Mental health › Epilepsy research and treatment
- Health Sciences › Medicine › Physiology › Tuberous Sclerosis Complex Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- parvalbumin, temporal lobe epilepsy, GABAergic system, inhibitory interneurons, epileptogenicity, calbindin, calretinin, neuropathology, immunocytochemistry
- MeSH terms
- Adolescent, Adult, Cell Count, Epilepsy, Temporal Lobe, Fluorescent Antibody Technique, Humans, Immunoenzyme Techniques, Immunohistochemistry, Interneurons, Microtubule-Associated Proteins, Middle Aged, Nerve Tissue Proteins, Parvalbumins, Phenothiazines, Staining and Labeling, Temporal Lobe, Microscopy, Confocal, Antigens, Nuclear, Calbindins, S100 Calcium Binding Protein G, Calbindin 2
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