Increased Expression of Mitochondrial Genes in Human Alcoholic Brain Revealed by Differential Display
Abstract
Polymerase chain reaction (PCR)‐based differential display was used to screen for alterations in gene expression in the mesolimbic system of the human alcoholic brain. Total RNA was extracted from the nucleus accumbens of five alcoholic and five control brains. A selected subpopulation of mRNA was reverse‐transcribed to cDNA and amplified by PCR. A differentially expressed cDNA fragment was recovered, cloned, and sequenced. Full sequence analysis of this 467 bp fragment revealed 98.2% homology with the human mitochondrial 125 rRNA gene. Dot‐blot analysis showed increased expression of this gene in nucleus accumbens and hippocampus, but not in the superior frontal cortex, primary motor cortex, caudate, and pallidus/putamen in a total of eight human alcoholic brains, compared with seven control brains. A similar increased expression was observed by dot‐blot analysis, using RNA from the cerebral cortex of rats chronically treated with alcohol vapor. Hybridization of a 16S rRNA oligonucleotide probe indicated that the expression of both rRNAs genes was significantly increased in nucleus accumbens. These results indicate that chronic alcohol consumption induces alteration in expression of mitochondrial genes in selected brain regions. The altered gene expression may reflect mitochondrial dysfunction in the alcohol‐affected brain.
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Alcohol exposure triggers changes in gene expression and biological pathways in human brain.
“Alcohol exposure triggers changes in gene expression and biological pathways in human brain.”
From Analysis of whole genome-transcriptomic organization in brain to identify genes associated with alcoholism (2019) · cites this paper as [5]
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- Authors
- Fan, L., van der Brug, M., Chen, W., Dodd, P. R., Matsumoto, I., Niwa, S., & Wilce, P. A.
- Affiliations
- Fukushima Medical University (JP)
- ORCID
- Dodd, Peter R.
- Venue
- Alcoholism: Clinical and Experimental Research
- Publisher
- Wiley
- Published
- 1999-03
- Volume
- 23
- Issue
- 3
- Pages
- 408-413
- ISSN
- 0145-6008 (ISSN-L)
- 1530-0277
- OpenAlex
- W2157440996
- Cited by
- 32
- References
- 25
- Citations by year
- 2026: 1 · 2020: 1 · 2019: 1 · 2015: 1 · 2014: 1 · 2012: 1
- FWCI
- 1.61
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Alcoholism Clinical and Experimental Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Health and Medical Research Council
- National Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Health Sciences › Medicine › Pathology and Forensic Medicine › Alcohol Consumption and Health Effects
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Keywords
- chronic alcohol consumption, mitochondrial gene expression, 12S rRNA, 16S rRNA, nucleus accumbens, hippocampus, mesolimbic system, mitochondrial dysfunction, alcohol-induced brain damage, differential gene expression
- MeSH terms
- Adult, Aged, Aged, 80 and over, Alcoholism, Animals, Brain, Cerebral Cortex, Cloning, Molecular, DNA, Mitochondrial, Female, Humans, Male, Middle Aged, Nucleus Accumbens, RNA, Messenger, RNA, Ribosomal, 16S, Blotting, Northern, Gene Expression, Rats, Wistar, Reverse Transcriptase Polymerase Chain Reaction, Rats
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