Reducing costs in flow-cytometric counting of residual white blood cells in blood products: utilization of a single-platform bead-free flow-rate calibration method

Fischer, Johannes C.; Quenzel, Ernst M.; Moog, Rainer; Wenzel, Folker; Riethmacher, Regina; Tutschek, Boris; Giers, Günther

doi:10.1111/j.1537-2995.2010.02998.x

Cited by 6Clinical Laboratory Practices and Quality ControlBlood transfusion and managementBlood donation and transfusion practices

Reducing costs in flow-cytometric counting of residual white blood cells in blood products: utilization of a single-platform bead-free flow-rate calibration method

Fischer, Johannes C., Quenzel, Ernst M., Moog, Rainer, Wenzel, Folker, Riethmacher, Regina, Tutschek, Boris, Giers, Günther·2011·Transfusion

DOI 10.1111/j.1537-2995.2010.02998.x

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Abstract

BACKGROUND: Commercial flow-cytometric methods for counting residual white blood cells (rWBCs) in leukoreduced blood products use calibration beads for estimation of the measured sample volume. A bead-free flow-rate calibration method is developed and validated. STUDY DESIGN AND METHODS: The analyzed volume was calculated by acquisition time (ACQ). Twenty-nine spiking series of red blood cell (RBC) or platelet (PLT) products were prepared containing levels ranging from 0.08 × 10(6) up to 2048 × 10(6) WBCs/L. Nearly WBC-free triple-leukofiltered RBCs or PLT concentrates (PCs) served as background. Propidium iodide (PI) was used to identify rWBCs. Five RBC series were compared against a commercially available kit (LeukoSure, Beckman Coulter). Routine capabilities were tested on 41 RBC and 92 PC samples of two independent transfusion services. RESULTS: The lower detection limit in RBC was 0.08 × 10(6) rWBCs/L for ACQ and 0.16 for LeukoSure. Criteria for linearity, accuracy, and precision were fulfilled within the range of 0.5 × 10(6) to 512 × 10(6) WBCs/L. For PCs, all these criteria were fulfilled between 0.5 × 10(6) and 32 × 10(6) rWBCs/L (lower detection limit of 0.25) for PI. ACQ and LeukoSure agreed sufficiently (81%) when tested on routine RBCs or PCs. CONCLUSION: A residual WBC count of fewer than 0.5 × 10(6) WBCs/L can be accurately counted using the ACQ approach at a total reagent cost of less than 0.5€ per sample.

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Authors: Fischer, J. C., Quenzel, E. M., Moog, R., Wenzel, F., Riethmacher, R., Tutschek, B., & Giers, G.
Affiliations: University Hospital of Bern (CH)
Corresponding author: Fischer, Johannes C.
ORCID: Fischer, Johannes C.
Quenzel, Ernst M.
Moog, Rainer
Tutschek, Boris
Venue: Transfusion
Publisher: Wiley
Published: 2011-01-07
Volume: 51
Issue: 7
Pages: 1431-1438
ISSN: 0041-1132 (ISSN-L)
1537-2995
DOI: 10.1111/j.1537-2995.2010.02998.x
OpenAlex: W2105393050
Cited by: 6
References: 41
Citations by year: 2018: 1 · 2015: 2 · 2014: 2 · 2012: 1
FWCI: 0.65
Type: journal article
Language: en
Open access: closed
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: Transfusion (publishedVersion)
PubMed (publishedVersion)
Topics: Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
Health Sciences › Medicine › Biochemistry › Blood transfusion and management
Social Sciences › Business, Management and Accounting › Management of Technology and Innovation › Blood donation and transfusion practices
Keywords: residual white blood cells, flow calibration, propidium iodide, packed red blood cells, platelet concentrates, leukocyte depletion, cost reduction, detection limit
MeSH terms: Calibration, Costs and Cost Analysis, Flow Cytometry, Humans, Leukocyte Count, Plateletpheresis, Quality Control, Erythrocyte Transfusion, Leukocyte Reduction Procedures, Limit of Detection

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Reducing costs in flow-cytometric counting of residual white blood cells in blood products: utilization of a single-platform bead-free flow-rate calibration method

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