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Cited by 71Yeasts and Rust Fungi StudiesFungal and yeast genetics researchPlant Pathogens and Fungal Diseases

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Molecular identification and genetic diversity within species of the genera Hanseniaspora and Kloeckera

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Cadez, Neza, Raspor, Peter, Cock, Arthur W.A.M., Boekhout, Teun, Smith, Maudy Th·2002·FEMS Yeast Research
DOI 10.1111/j.1567-1364.2002.tb00046.x
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Abstract

Three molecular methods, RAPD-PCR analysis, electrophoretic karyotyping and RFLP of the PCR-amplified ITS regions (ITS1, ITS2 and the intervening 5.8S rDNA), were studied for accurate identification of Hanseniaspora and Kloeckera species as well as for determining inter- and intraspecific relationships of 74 strains isolated from different sources and/or geographically distinct regions. Of these three methods, PCR-RFLP analysis of ITS regions with restriction enzymes DdeI and HinfI is proposed as a rapid identification method to discriminate unambiguously between all six Hanseniaspora species and the single non-ascospore-forming apiculate yeast species Kloeckera lindneri. Electrophoretic karyotyping produced chromosomal profiles by which the seven species could be divided into four groups sharing similar karyotypes. Although most of the 60 strains examined exhibited a common species-specific pattern, a different degree of chromosomal-length polymorphism and a variable number of chromosomal DNA fragments were observed within species. Cluster analysis of the combined RAPD-PCR fingerprints obtained with one 10-mer primer, two microsatellite primers and one minisatellite primer generated clusters which with a few exceptions are in agreement with the groups as earlier recognized in DNA-DNA homology studies.

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Metadata

Authors
Cadez, N., Raspor, P., Cock, A. W. A. M., Boekhout, T., & Smith, M. T.
Affiliations
  • University of Ljubljana (SI)
  • Westerdijk Fungal Biodiversity Institute (NL)
ORCID
Venue
FEMS Yeast Research
Publisher
Oxford University Press (OUP)
Published
2002-01
Volume
1
Issue
4
Pages
279-289
ISSN
  • 1567-1356 (ISSN-L)
  • 1567-1364
DOI
10.1111/j.1567-1364.2002.tb00046.x
OpenAlex
W2103713935
Cited by
71
References
0
Citations by year
2026: 1 · 2023: 1 · 2021: 3 · 2020: 4 · 2019: 5 · 2018: 3 · 2017: 6 · 2016: 3 · 2015: 6 · 2014: 3 · 2013: 2 · 2012: 2
FWCI
1.04
Type
journal article
Language
en
Open access
Locations
  • FEMS Yeast Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Microbiology › Yeasts and Rust Fungi Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Pathogens and Fungal Diseases
Keywords
Hanseniaspora, Kloeckera, yeast identification, genetic diversity, ITS region, PCR-RFLP, electrophoretic karyotyping, RAPD-PCR, chromosomal polymorphism, microsatellite primers, clustering
MeSH terms
Animals, Ascomycota, DNA, Fungal, DNA, Ribosomal, Saccharomycetales, Humans, Karyotyping, Polymorphism, Restriction Fragment Length, Species Specificity, Genetic Variation, Deoxyribonucleases, Type II Site-Specific, Electrophoresis, Gel, Pulsed-Field, Mycological Typing Techniques, Random Amplified Polymorphic DNA Technique

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