The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Introduction and expression of genes for metabolic engineering applications in Saccharomyces cerevisiae
Open accessAbstract
Metabolic pathway engineering in the yeast Saccharomyces cerevisiae leads to improved production of a wide range of compounds, ranging from ethanol (from biomass) to natural products such as sesquiterpenes. The introduction of multienzyme pathways requires precise control over the level and timing of expression of the associated genes. Gene number and promoter strength/regulation are two critical control points, and multiple studies have focused on modulating these in yeast. This MiniReview focuses on methods for introducing genes and controlling their copy number and on the many promoters (both constitutive and inducible) that have been successfully employed. The advantages and disadvantages of the methods will be presented, and applications to pathway engineering will be highlighted.
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Cites
Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
Gossen, M., & Bujard, H. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.12.5547 · Cited by 4,943
Production of the antimalarial drug precursor artemisinic acid in engineered yeast
Ro, D.-K., Paradise, E. M., Ouellet, M., Fisher, K. J., Newman, K. L., Ndungu, J. M., Ho, K. A., Eachus, R. A., Ham, T. S., Kirby, J., et al. (2006) · Nature · doi:10.1038/nature04640 · Cited by 2,805
Transcriptional Activation by Tetracyclines in Mammalian Cells
Gossen, M., Freundlieb, S., Bender, G., Müller, G., Hillen, W., & Bujard, H. (1995) · Science · doi:10.1126/science.7792603 · Cited by 2,488 · cited 1×
“One of the most useful heterologous promoter systems in yeast utilizes the bacterial tetracycline operator (tetO) and hybrid transactivator, based on the expression system developed for mammalian cells (Gossen & Bujard, 1992; Gossen et al., 1995) and shown to be active in yeast by Dingermann et al.”
BackgroundEngineering Yeast Transcription Machinery for Improved Ethanol Tolerance and Production
Alper, H., Moxley, J., Nevoigt, E., Fink, G. R., & Stephanopoulos, G. (2006) · Science · doi:10.1126/science.1131969 · Cited by 823
Sauer, B. (1987) · Molecular and Cellular Biology · doi:10.1128/mcb.7.6.2087 · Cited by 482
Wisselink, H. W., Toirkens, M. J., del Rosario Franco Berriel, M., Winkler, A. A., van Dijken, J. P., Pronk, J. T., & van Maris, A. J. A. (2007) · Applied and Environmental Microbiology · doi:10.1128/aem.00177-07 · Cited by 209
Ro, D.-K., Ouellet, M., Paradise, E. M., Burd, H., Eng, D., Paddon, C. J., Newman, J. D., & Keasling, J. D. (2008) · BMC Biotechnology · doi:10.1186/1472-6750-8-83 · Cited by 197
Cited by
A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
Lee, M. E., DeLoache, W. C., Cervantes, B., & Dueber, J. E. (2015) · ACS Synthetic Biology · doi:10.1021/sb500366v · Cited by 916
Metadata
- Authors
- Da Silva, N. A., & Srikrishnan, S.
- Venue
- FEMS Yeast Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2012-01-12
- Volume
- 12
- Issue
- 2
- Pages
- 197-214
- ISSN
- 1567-1356 (ISSN-L)
- OpenAlex
- W2083149221
- Cited by
- 260
- References
- 7
- FWCI
- 3.88
- Type
- journal article
- Language
- en
- Open access
- bronze
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