Cited by 482Fungal and yeast genetics researchCRISPR and Genetic EngineeringDNA Repair Mechanisms

Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae

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Sauer, B·1987·Molecular and Cellular Biology

Abstract

The procaryotic cre-lox site-specific recombination system of coliphage P1 was shown to function in an efficient manner in a eucaryote, the yeast Saccharomyces cerevisiae. The cre gene, which codes for a site-specific recombinase, was placed under control of the yeast GALI promoter. lox sites flanking the LEU2 gene were integrated into two different chromosomes in both orientations. Excisive recombination at the lox sites (as measured by loss of the LEU2 gene) was promoted efficiently and accurately by the Cre protein and was dependent upon induction by galactose. These results demonstrate that a procaryotic recombinase can enter a eucaryotic nucleus and, moreover, that the ability of the Cre recombinase to perform precise recombination events on the chromosomes of S. cerevisiae is unimpaired by chromatin structure.

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Crossref · 10.1128/mcb.7.6.2087

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Authors
Sauer, B.
Affiliations
DuPont (United States) (US)
Corresponding author
Sauer, B
Venue
Molecular and Cellular Biology
Published
1987-06
Volume
7
Issue
6
Pages
2087-2096
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
OpenAlex
W1913334531
Cited by
482
References
38
Citations by year
2025: 11 · 2024: 9 · 2023: 15 · 2022: 17 · 2021: 17 · 2020: 15 · 2019: 20 · 2018: 27 · 2017: 15 · 2016: 19 · 2015: 21 · 2014: 34 · 2013: 18 · 2012: 21
FWCI
1.79
Type
journal article
Language
en
Open access
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
Keywords
Cre recombinase, Saccharomyces cerevisiae, bacteriophage P1, excision, LEU2 gene, GAL1 promoter, galactose induction, chromatin structure, chromosomal integration
MeSH terms
Coliphages, DNA Nucleotidyltransferases, DNA Restriction Enzymes, Escherichia coli, Genes, Genes, Fungal, Genes, Viral, Plasmids, Promoter Regions, Genetic, Recombination, Genetic, Saccharomyces cerevisiae, Viral Proteins, Integrases

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