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Cited by 15Escherichia coli research studiesProbiotics and Fermented FoodsClostridium difficile and Clostridium perfringens research

The DOI matches its Crossref record; 56 of the 56 references it cites were found in a registry or an index.

HlyA knock out yields a safer Escherichia coli A0 34/86 variant with unaffected colonization capacity in piglets

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Sheshko, Valeria, Hejnova, Jana, Rehakova, Zuzana, Sinkora, Jiri, Faldyna, Martin, Alexa, Pavel, Felsberg, Jurgen, Nemcova, Radomira, Bomba, Alojz, Sebo, Peter·2006·FEMS Immunology & Medical Microbiology
DOI 10.1111/j.1574-695x.2006.00140.x
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Abstract

Escherichia coli A0 34/86 (O83:K24:H31) has been successfully used for prophylactic and therapeutic intestinal colonization of premature and newborn infants, with the aim of preventing nosocomial infections. Although E. coli A0 34/86 was described as a nonpathogenic commensal, partial sequencing revealed that its genome harbours gene clusters highly homologous to virulence determinants of different types of E. coli, including closely linked genes of the alpha-haemolysin operon (hlyCABD) and for the cytotoxic necrotizing factor (cnf1). A haemolysin-deficient mutant (Delta hlyA) of E. coli A0 34/86 was generated and its colonization capacity was determined. The results show that a single dose of the A0 34/86 wild-type or Delta hlyA strains resulted in efficient intestinal colonization of newborn conventional piglets, and that this was still considerable after several weeks. No difference was observed between the wild-type and the mutant strains, showing that haemolysin expression does not contribute to intestinal colonization capacity of E. coli A0 34/86. Safety experiments revealed that survival of colostrum-deprived gnotobiotic newborn piglets was substantially higher upon colonization by the nonhaemolytic strain than following inoculation by its wild-type ancestor. We suggest that the E. coli A0 34/86 Delta hlyA mutant may represent a safer prophylactic and/or immunomodulatory tool with unaffected colonization capacity.

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Metadata

Authors
Sheshko, V., Hejnova, J., Rehakova, Z., Sinkora, J., Faldyna, M., Alexa, P., Felsberg, J., Nemcova, R., Bomba, A., & Sebo, P.
Affiliations
  • Czech Academy of Sciences (CZ)
  • Veterinary Research Institute (CZ)
  • University of Veterinary Medicine in Košice (SK)
ORCID
Venue
FEMS Immunology & Medical Microbiology
Publisher
Oxford University Press (OUP)
Published
2006-11
Volume
48
Issue
2
Pages
257-266
ISSN
  • 0928-8244 (ISSN-L)
  • 1574-695X
DOI
10.1111/j.1574-695x.2006.00140.x
OpenAlex
W2010793151
Cited by
15
References
56
Citations by year
2025: 2 · 2024: 1 · 2022: 1 · 2018: 2 · 2016: 1 · 2015: 3 · 2013: 1 · 2012: 1
FWCI
0.20
Type
journal article
Language
en
Open access
Locations
  • FEMS Immunology & Medical Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Grantová Agentura České Republiky (AV0Z50200510)
  • Sixth Framework Programme
Topics
  • Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Health Sciences › Medicine › Infectious Diseases › Clostridium difficile and Clostridium perfringens research
Keywords
alpha-hemolysin, intestinal colonization, piglets, gnotobiotic pigs, colostrum deprivation, bacterial safety, nosocomial infection prevention, cytotoxic necrotizing factor, virulence genes
MeSH terms
Animals, Bacterial Toxins, Chromosome Mapping, Escherichia coli, Escherichia coli Infections, Hemolysin Proteins, Genetic Linkage, Swine, Swine Diseases, Swine, Miniature, Gene Deletion, Escherichia coli Proteins

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