The DOI matches its Crossref record; 56 of the 56 references it cites were found in a registry or an index.
HlyA knock out yields a safer Escherichia coli A0 34/86 variant with unaffected colonization capacity in piglets
Open accessAbstract
Escherichia coli A0 34/86 (O83:K24:H31) has been successfully used for prophylactic and therapeutic intestinal colonization of premature and newborn infants, with the aim of preventing nosocomial infections. Although E. coli A0 34/86 was described as a nonpathogenic commensal, partial sequencing revealed that its genome harbours gene clusters highly homologous to virulence determinants of different types of E. coli, including closely linked genes of the alpha-haemolysin operon (hlyCABD) and for the cytotoxic necrotizing factor (cnf1). A haemolysin-deficient mutant (Delta hlyA) of E. coli A0 34/86 was generated and its colonization capacity was determined. The results show that a single dose of the A0 34/86 wild-type or Delta hlyA strains resulted in efficient intestinal colonization of newborn conventional piglets, and that this was still considerable after several weeks. No difference was observed between the wild-type and the mutant strains, showing that haemolysin expression does not contribute to intestinal colonization capacity of E. coli A0 34/86. Safety experiments revealed that survival of colostrum-deprived gnotobiotic newborn piglets was substantially higher upon colonization by the nonhaemolytic strain than following inoculation by its wild-type ancestor. We suggest that the E. coli A0 34/86 Delta hlyA mutant may represent a safer prophylactic and/or immunomodulatory tool with unaffected colonization capacity.
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“Noteworthy, E. coli A0 34/86, a strain apparently devoid of the pks gene [47], contains at least two potential pathogenic genes (hly, cnf-1) respectively responsible for alpha-hemolysin and cytotoxic necrotizing factor-1 production and release [48].”
From Selection, Comparative Genomics, and Potential Probiotic Features of Escherichia coli 5C, a pks-Negative Strain Isolated from Healthy Infant Donor Feces (2026) · cites this paper as [48]
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Di Pierro, F., Sagheddu, V., Galletti, S., Casaroli, A., Labrini, E., Soldi, S., Cazzaniga, M., Bertuccioli, A., Matera, M., Cavecchia, I., et al. (2026) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-025-10522-5 · Cited by 5
“Noteworthy, E. coli A0 34/86, a strain apparently devoid of the pks gene [47], contains at least two potential pathogenic genes ( hly , cnf-1 ) respectively responsible for alpha-hemolysin and cytotoxic necrotizing factor-1 production and release [48].”
Metadata
- Authors
- Sheshko, V., Hejnova, J., Rehakova, Z., Sinkora, J., Faldyna, M., Alexa, P., Felsberg, J., Nemcova, R., Bomba, A., & Sebo, P.
- Affiliations
- Czech Academy of Sciences (CZ)
- Veterinary Research Institute (CZ)
- University of Veterinary Medicine in Košice (SK)
- Venue
- FEMS Immunology & Medical Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-11
- Volume
- 48
- Issue
- 2
- Pages
- 257-266
- ISSN
- 0928-8244 (ISSN-L)
- 1574-695X
- OpenAlex
- W2010793151
- Cited by
- 15
- References
- 56
- Citations by year
- 2025: 2 · 2024: 1 · 2022: 1 · 2018: 2 · 2016: 1 · 2015: 3 · 2013: 1 · 2012: 1
- FWCI
- 0.20
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEMS Immunology & Medical Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Grantová Agentura České Republiky (AV0Z50200510)
- Sixth Framework Programme
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Medicine › Infectious Diseases › Clostridium difficile and Clostridium perfringens research
- Keywords
- alpha-hemolysin, intestinal colonization, piglets, gnotobiotic pigs, colostrum deprivation, bacterial safety, nosocomial infection prevention, cytotoxic necrotizing factor, virulence genes
- MeSH terms
- Animals, Bacterial Toxins, Chromosome Mapping, Escherichia coli, Escherichia coli Infections, Hemolysin Proteins, Genetic Linkage, Swine, Swine Diseases, Swine, Miniature, Gene Deletion, Escherichia coli Proteins
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