The DOI matches its Crossref record; 27 of the 30 references it cites were found in a registry or an index, and 1 are not checked yet.
The filamentation pathway controlled by the Efg1 regulator protein is required for normal biofilm formation and development in Candida albicans
Abstract
Candida albicans biofilms are structured microbial communities composed of a mixture of yeast cells and hyphal elements, suggesting a pivotal role for the dimorphic switch in the development of biofilms. We have used C. albicans mutants defective in genes involved in filamentation (Δcph1, Δefg1, Δhst7, and Δcst20) and compared these mutants to wild-type strains to determine whether filamentation is an integral factor for biofilm formation. Scanning electron microscopy revealed that Δcph1, Δhst7 and Δcst20 mutants were able to filament and form structured biofilms displaying three-dimensional architecture similar to those formed by wild-type strains. However, Δefg1 and Δcph1/Δefg1 mutants were unable to filament and did not form biofilms, but rather sparse monolayers of loosely attached elongated, rod-like, cells. Antimicrobial susceptibility testing showed intrinsic resistance of all mutant strains to fluconazole and amphotericin B when attached to the surface of biomaterials. These results suggest that hyphal formation is pivotal for biofilm development in C. albicans. However, the sessile lifestyle associated with adherent cells confers antifungal resistance, regardless of coherent biofilm formation.
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“Although they readily form in planktonic cultures, hyphae are an important structural component of C. albicans biofilms; thus, it is not surprising that genes required for hyphal growth in suspension cultures are also necessary for proper biofilm formation.”
From Candida albicans Biofilms and Human Disease (2015) · cites this paper as [163]
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Nonfilamentous C. albicans Mutants Are Avirulent
Lo, H.-J., Köhler, J. R., DiDomenico, B., Loebenberg, D., Cacciapuoti, A., & Fink, G. R. (1997) · Cell · doi:10.1016/s0092-8674(00)80358-x · Cited by 1,809
Isogenic strain construction and gene mapping in Candida albicans.
Fonzi, W. A., & Irwin, M. Y. (1993) · Genetics · doi:10.1093/genetics/134.3.717 · Cited by 1,695
Gimeno, C. J., Ljungdahl, P. O., Styles, C. A., & Fink, G. R. (1992) · Cell · doi:10.1016/0092-8674(92)90079-r · Cited by 1,180
Suppression of Hyphal Formation in Candida albicans by Mutation of a STE12 Homolog
Liu, H., Köhler, J. R., & Fink, G. R. (1994) · Science · doi:10.1126/science.7992058 · Cited by 788
Standardized Method for In Vitro Antifungal Susceptibility Testing of Candida albicans Biofilms
Ramage, G., Vande Walle, K., Wickes, B. L., & López-Ribot, J. L. (2001) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.45.9.2475-2479.2001 · Cited by 753
Self-citationRoberts, R. L., & Fink, G. R. (1994) · Genes & Development · doi:10.1101/gad.8.24.2974 · Cited by 616
Stoldt, V. R., Sonneborn, A., Leuker, C. E., & Ernst, J. F. (1997) · The EMBO Journal · doi:10.1093/emboj/16.8.1982 · Cited by 595
Elements of the Yeast Pheromone Response Pathway Required for Filamentous Growth of Diploids
Liu, H., Styles, C. A., & Fink, G. R. (1993) · Science · doi:10.1126/science.8259520 · Cited by 500
Ramage, G. (2002) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkf049 · Cited by 468
Self-citationRas Signaling Is Required for Serum-Induced Hyphal Differentiation in Candida albicans
Feng, Q., Summers, E., Guo, B., & Fink, G. R. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.20.6339-6346.1999 · Cited by 347
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2 of the 30 references couldn’t be matched to a known paper.
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Sardi, J. C. O., Scorzoni, L., Bernardi, T., Fusco-Almeida, A. M., & Mendes Giannini, M. J. S. (2013) · Journal of Medical Microbiology · doi:10.1099/jmm.0.045054-0 · Cited by 1,184
Candida biofilms and their role in infection
Douglas, L. J. (2003) · Trends in Microbiology · doi:10.1016/s0966-842x(02)00002-1 · Cited by 1,150
Candida albicans Biofilms and Human Disease
Nobile, C. J., & Johnson, A. D. (2015) · Annual Review of Microbiology · doi:10.1146/annurev-micro-091014-104330 · Cited by 1,057
Candida albicans biofilms: development, regulation, and molecular mechanisms
Gulati, M., & Nobile, C. J. (2016) · Microbes and Infection · doi:10.1016/j.micinf.2016.01.002 · Cited by 738
A Recently Evolved Transcriptional Network Controls Biofilm Development in Candida albicans
Nobile, C. J., Fox, E. P., Nett, J. E., Sorrells, T. R., Mitrovich, Q. M., Hernday, A. D., Tuch, B. B., Andes, D. R., & Johnson, A. D. (2012) · Cell · doi:10.1016/j.cell.2011.10.048 · Cited by 711
Metadata
- Authors
- Ramage, G., VandeWalle, K., López-Ribot, J. L., & Wickes, B. L.
- Affiliations
- The University of Texas at San Antonio Health Science Center (US)
- Corresponding author
- Wickes, Brian L
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-08
- Volume
- 214
- Issue
- 1
- Pages
- 95-100
- ISSN
- 0378-1097 (ISSN-L)
- 1574-6968
- OpenAlex
- W1981442035
- Cited by
- 334
- References
- 30
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 15 · 2023: 13 · 2022: 14 · 2021: 21 · 2020: 12 · 2019: 23 · 2018: 11 · 2017: 14 · 2016: 20 · 2015: 25 · 2014: 26 · 2013: 12 · 2012: 17
- FWCI
- 5.08
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEMS Microbiology Letters (publishedVersion)
- PubMed (publishedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (submittedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Keywords
- Candida albicans, biofilm formation, hyphal development, antifungal resistance, fluconazole resistance, amphotericin B resistance, Cph1, yeast-to-hypha transition
- MeSH terms
- Amphotericin B, Antifungal Agents, Candida albicans, Culture Media, DNA-Binding Proteins, Fungal Proteins, Microbial Sensitivity Tests, Microscopy, Electron, Scanning, Transcription Factors, Fluconazole, Gene Expression Regulation, Fungal, Biofilms
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