The lipoprotein components of the Isd and Hts transport systems are dispensable for acquisition of heme by Staphylococcus aureus

Wright, John A.; Nair, Sean P.

doi:10.1111/j.1574-6968.2012.02519.x

Cited by 17Antimicrobial Resistance in StaphylococcusBacterial Genetics and BiotechnologyHemoglobin structure and function

The lipoprotein components of the Isd and Hts transport systems are dispensable for acquisition of heme by Staphylococcus aureus

Wright, John A., Nair, Sean P.·2012·FEMS Microbiology Letters

DOI 10.1111/j.1574-6968.2012.02519.x

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Abstract

Heme is a key molecule for Staphylococcus aureus and is involved in many aspects of oxidative metabolism. Crucially, heme is required for the activity of cytochromes of the electron transport chain. Staphylococcus aureus is able to obtain heme either through biosynthesis or through acquisition from the host. Clinically persistent 'small colony variant' (SCV) forms of S. aureus are frequently deficient for heme biosynthesis, and disruption of the hemB gene produces stable heme-auxotrophic strains that reproduce many SCV phenotypes. We sought to address the role of heme transport in SCVs by deleting components of the two described heme import systems, the iron-regulated surface determinant (Isd) and heme transport system (Hts) in wild-type S. aureus and hemB mutant backgrounds. Analysis of the growth of S. aureus hemB strains either singly or doubly deficient in isdE and htsA in the presence and absence of heme or hemoglobin revealed that S. aureus is able to obtain exogenous heme in the absence of these transporter components. These data suggest the presence of additional, as yet unidentified transporter components that enable S. aureus to internalize exogenous heme and contradict the proposed model that IsdE can transfer heme to the HtsBC permease.

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Two recent reports challenged the large literature on the Isd system's role in hemoglobin/heme-derived iron acquisition.
“However, the wealth of literature on the contribution of the Isd system to hemoglobin/heme-derived iron acquisition was challenged by 2 recent reports [36, 37].”
From IsdB-dependent hemoglobin binding is required for acquisition of heme by Staphylococcus aureus (2014) · cites this paper as [37]

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IsdB-dependent hemoglobin binding is required for acquisition of heme by Staphylococcus aureus
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Authors: Wright, J. A., & Nair, S. P.
Affiliations: University College London (GB)
Corresponding author: Nair, Sean P.
ORCID: Wright, John A.
Nair, Sean P.
Venue: FEMS Microbiology Letters
Publisher: Oxford University Press (OUP)
Published: 2012-02-23
Volume: 329
Issue: 2
Pages: 177-185
ISSN: 0378-1097 (ISSN-L)
DOI: 10.1111/j.1574-6968.2012.02519.x
OpenAlex: W2018849855
Cited by: 17
References: 49
Citations by year: 2025: 2 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 2 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 2 · 2012: 1
FWCI: 1.33
Type: journal article
Language: en
Open access: closed
Locations: FEMS Microbiology Letters (publishedVersion)
PubMed (publishedVersion)
UCL Discovery (University College London) (submittedVersion)
Funders: Versus Arthritis (18294)
Topics: Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Hemoglobin structure and function
Keywords: Staphylococcus aureus, heme acquisition, small colony variants, hemoglobin, bacterial transporters
MeSH terms: Porphobilinogen Synthase, Bacterial Proteins, Biological Transport, Active, Carrier Proteins, Culture Media, Escherichia coli, Heme, Humans, Lipoproteins, Mutation, Phenotype, Staphylococcus aureus, Gene Deletion

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The lipoprotein components of the Isd and Hts transport systems are dispensable for acquisition of heme by Staphylococcus aureus

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