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In vitro differentiation of human mesenchymal stem cells to epithelial lineage
Open accessAbstract
Our study examined whether human bone marrow‐derived MSCs are able to differentiate, in vitro, into functional epithelial‐like cells. MSCs were isolated from the sternum of 8 patients with different hematological disorders. The surface phenotype of these cells was characterized.To induce epithelial differentiation, MSCs were cultured using Epidermal Growth Factor, Keratinocyte Growth Factor, Hepatocyte Growth Factor and Insulin‐like growth Factor‐II. Differentiated cells were further characterized both morphologically and functionally by their capacity to express markers with specificity for epithelial lineage. The expression of cytokeratin 19 was assessed by immunocytochemistry, and cytokeratin 18 was evaluated by quantitative RT‐PCR (Taq‐man). The data demonstrate that human MSCs isolated from human bone marrow can differentiate into epithelial‐like cells and may thus serve as a cell source for tissue engineering and cell therapy of epithelial tissue.
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Gage, F. H. (2000) · Science · doi:10.1126/science.287.5457.1433 · Cited by 4,786
Cited by
Mesenchymal Stem Cells for Regenerative Medicine
Han, Y., Li, X., Zhang, Y., Han, Y., Chang, F., & Ding, J. (2019) · Cells · doi:10.3390/cells8080886 · Cited by 1,093
Therapeutic Potential of Mesenchymal Stem Cells for Cancer Therapy
Hmadcha, A., Martin-Montalvo, A., Gauthier, B. R., Soria, B., & Capilla-Gonzalez, V. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00043 · Cited by 341
Yadav, P., Vats, R., Bano, A., & Bhardwaj, R. (2020) · Life Sciences · doi:10.1016/j.lfs.2020.118588 · Cited by 36
Metadata
- Authors
- Păunescu, V., Deak, E., Herman, D., Siska, I. R., T˘anasie, G., Bunu, C., Anghel, S., Tatu, C. A., Oprea, T. I., Henschler, R., Rüster, B., Bistrian, R., & Seifried, E.
- Venue
- Journal of Cellular and Molecular Medicine
- Publisher
- Wiley
- Published
- 2007-05
- Volume
- 11
- Issue
- 3
- Pages
- 502-508
- ISSN
- 1582-1838 (ISSN-L)
- 1582-4934
- OpenAlex
- W2044193051
- Cited by
- 165
- References
- 1
- FWCI
- 2.12
- Type
- journal article
- Language
- en
- Open access
- bronze
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- The paper
- Păunescu, V., Deak, E., Herman, D., Siska, I. R., T˘anasie, G., Bunu, C., Anghel, S., Tatu, C. A., Oprea, T. I., Henschler, R., Rüster, B., Bistrian, R., & Seifried, E. (2007). In vitro differentiation of human mesenchymal stem cells to epithelial lineage. Journal of Cellular and Molecular Medicine. https://doi.org/10.1111/j.1582-4934.2007.00041.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
