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Using optical coherence tomography for the longitudinal non-invasive evaluation of epidermal thickness in a murine model of chronic skin inflammation
Open accessAbstract
Background: Non‐invasive methods are desirable for longitudinal studies examining drug efficacy and disease resolution defined as decreases in epidermal thickness in mouse models of psoriasiform skin disease. This would eliminate the need for either sacrificing animals or collecting serial skin biopsies to evaluate changes in disease progression during an individual study. The quantitation of epidermal thickness using optical coherence tomography (OCT) provides an alternative to traditional histology techniques. Methods: Using the KC‐Tie2 doxycycline‐repressible psoriasiform skin disease mouse model, OCT imaging was completed on diseased back skin of adult KC‐Tie2 (n = 3–4) and control (n = 3–4) mice, followed immediately by the surgical excision of the same region for histologic analyses. Animals were then treated with doxycycline to suppress transgene expression and to reverse the skin disease and additional OCT images and tissues were collected 2 and 4 weeks following. Epidermal thickness was measured using OCT and histology. Results: Optical coherence tomography and histology both demonstrated that KC‐Tie2 mice had significantly thicker epidermis (~4‐fold; P < 0.0001) than control animals. By 2 weeks following gene repression, decreases in epidermal thickness were observed using both OCT and histology, and were sustained through 4 weeks. Correlation analyses between histology and OCT values at all time points and in all animals revealed high significance (R2 = 0.78); with correlation being highest in KC‐Tie2 mice (R2 = 0.92) compared to control animals (R2 = 0.16). Conclusion: Non‐invasive OCT imaging provided similar values as those collected using standard histologic measures in thick skin of KC‐Tie2 mice but became less reliable in thinner control mouse skin, possibly reflecting limitations in resolution of OCT. Future advances in resolution of OCT may improve and allow greater accuracy of epidermal thickness measurements.
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We used optical coherence tomography, which strongly correlates with histological measurements.
“We used optical coherence tomography, which strongly correlates with histological measurements (Silver et al, 2012).”
From Effect of Repeated Low-Dose UVR Exposure on Skin Inflammation Threshold, Skin Biomarkers, and Vitamin D in Healthy Adults (2025) · cites this paper as Silver et al, 2012
The Results report a strong correlation between OCT and histological epidermal-thickness measurements across the mice studied (R = 0.8827), and an even stronger correlation in KC-Tie2 mice. The unqualified claim overlooks that the correlation in thin-skinned control mice was weaker and not statistically significant (R = 0.4205, p = 0.2276). (LLM, conf=0.87)
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de Boer, F., Kezic, S., van der Lelie, G., Motazedi, E., Rustemeyer, T., van Dijk, A., Almasian, M., Jakasa, I., & van der Molen, H. F. (2025) · Journal of Investigative Dermatology · doi:10.1016/j.jid.2025.01.024 · Cited by 4
Metadata
- Authors
- Silver, R., Helms, A., Fu, W., Wang, H., Diaconu, D., Loyd, C. M., Rollins, A. M., & Ward, N. L.
- Affiliations
- Case Western Reserve University (US)
- University Hospitals of Cleveland (US)
- Corresponding author
- Ward, Nicole L.
- Venue
- Skin Research and Technology
- Publisher
- Wiley
- Published
- 2011-09-14
- Volume
- 18
- Issue
- 2
- Pages
- 225-231
- ISSN
- 0909-752X (ISSN-L)
- 1600-0846
- PMC
- PMC3319809
- OpenAlex
- W2059115311
- Cited by
- 16
- References
- 31
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2016: 2 · 2015: 2 · 2013: 1 · 2012: 1
- FWCI
- 0.38
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Skin Research and Technology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Cancer Institute (R01CA114276)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (P50AR055508, P30AR039750, P50AR05508, P30 AR39750)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Optical Coherence Tomography Applications
- Life Sciences › Immunology and Microbiology › Immunology › Psoriasis: Treatment and Pathogenesis
- Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
- Keywords
- optical coherence tomography, epidermal thickness, chronic skin inflammation, non-invasive imaging, histopathological correlation, disease resolution
- MeSH terms
- Animals, Anti-Bacterial Agents, Biopsy, Chronic Disease, Dermatitis, Disease Models, Animal, Doxycycline, Epidermis, Longitudinal Studies, Mice, Mutant Strains, Psoriasis, Sensitivity and Specificity, Severity of Illness Index, Reproducibility of Results, Keratinocytes, Gene Expression, Transgenes, Receptor Protein-Tyrosine Kinases, Tomography, Optical Coherence, Receptor, TIE-2, Mice
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