Space, time and nitric oxide – neuronal nitric oxide synthase generates signal pulses
Abstract
The temporal aspects of signaling are critical to the function of signals in communications, feedback regulation and control. The production and transduction of biological signals by enzymes comprises an area of central importance and rapid progress in the biomedical sciences. Treatment of signaling enzymes almost universally employs steady‐state analyses that are suitable for mass catalysis but inappropriate for components in an information channel or a feedback/control system. In the present study, we show that, at 37 °C, neuronal nitric oxide synthase (EC 1.14.13.39) is progressively inhibited by the formation of an inhibited state during the first few turnovers (approximately 200 ms) after the initiation of catalysis, leading to pulse formation of nitric oxide. The general mechanism may be of wide importance in biological signaling.
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Mitochondrial ROS have been shown to induce organellar degradation (e.g., mitophagy) under specific conditions.
“As a counterbalance, mitochondrial ROS have also been shown to induce organellar degradation (e.g., mitophagy) under specific conditions (810).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [810]
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Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation
Tejero, J., Shiva, S., & Gladwin, M. T. (2019) · Physiological Reviews · doi:10.1152/physrev.00036.2017 · Cited by 541 · see citing works
- Authors
- Salerno, J. C., & Ghosh, D. K.
- Affiliations
- Kennesaw State University (US)
- Duke University (US)
- ORCID
- Ghosh, Dipak K.
- Venue
- The FEBS Journal
- Publisher
- Wiley
- Published
- 2009-10-23
- Volume
- 276
- Issue
- 22
- Pages
- 6677-6688
- ISSN
- 1742-464X (ISSN-L)
- 1742-4658
- OpenAlex
- W1530399633
- Cited by
- 21
- References
- 45
- Citations by year
- 2021: 1 · 2020: 1 · 2019: 4 · 2018: 2 · 2016: 2 · 2015: 1 · 2012: 1
- FWCI
- 2.21
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEBS Journal (publishedVersion)
- PubMed (publishedVersion)
- DigitalCommons - Kennesaw State University (Kennesaw State University) (submittedVersion)
- Funders
- National Institutes of Health (1r15gm083317-01)
- National Institute of General Medical Sciences (GM083317-01)
- Topics
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- neuronal nitric oxide synthase, nitric oxide, enzyme inhibition, transient inhibition, enzyme kinetics, biological signaling, feedback regulation
- MeSH terms
- Animals, Kinetics, Models, Theoretical, Nitric Oxide, Signal Transduction, Rats, Nitric Oxide Synthase Type I
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