Cited by 21Nitric Oxide and Endothelin EffectsReceptor Mechanisms and SignalingRedox biology and oxidative stress

Space, time and nitric oxide – neuronal nitric oxide synthase generates signal pulses

Salerno, John C., Ghosh, Dipak K.·2009·The FEBS Journal

Abstract

The temporal aspects of signaling are critical to the function of signals in communications, feedback regulation and control. The production and transduction of biological signals by enzymes comprises an area of central importance and rapid progress in the biomedical sciences. Treatment of signaling enzymes almost universally employs steady‐state analyses that are suitable for mass catalysis but inappropriate for components in an information channel or a feedback/control system. In the present study, we show that, at 37 °C, neuronal nitric oxide synthase (EC 1.14.13.39) is progressively inhibited by the formation of an inhibited state during the first few turnovers (approximately 200 ms) after the initiation of catalysis, leading to pulse formation of nitric oxide. The general mechanism may be of wide importance in biological signaling.

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Crossref · 10.1111/j.1742-4658.2009.07382.x

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45 of 45 references verified0 citation errors · 0 not checkable

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Authors
Salerno, J. C., & Ghosh, D. K.
Affiliations
  • Kennesaw State University (US)
  • Duke University (US)
Venue
The FEBS Journal
Publisher
Wiley
Published
2009-10-23
Volume
276
Issue
22
Pages
6677-6688
ISSN
  • 1742-464X (ISSN-L)
  • 1742-4658
OpenAlex
W1530399633
Cited by
21
References
45
Citations by year
2021: 1 · 2020: 1 · 2019: 4 · 2018: 2 · 2016: 2 · 2015: 1 · 2012: 1
FWCI
2.21
Type
journal article
Language
en
Open access
closed
Locations
  • FEBS Journal (publishedVersion)
  • PubMed (publishedVersion)
  • DigitalCommons - Kennesaw State University (Kennesaw State University) (submittedVersion)
Funders
  • National Institutes of Health (1r15gm083317-01)
  • National Institute of General Medical Sciences (GM083317-01)
Topics
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords
neuronal nitric oxide synthase, nitric oxide, enzyme inhibition, transient inhibition, enzyme kinetics, biological signaling, feedback regulation
MeSH terms
Animals, Kinetics, Models, Theoretical, Nitric Oxide, Signal Transduction, Rats, Nitric Oxide Synthase Type I

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