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Macromolecular NMR spectroscopy for the non‐spectroscopist
Abstract
NMR spectroscopy is a powerful tool for studying the structure, function and dynamics of biological macromolecules. However, non‐spectroscopists often find NMR theory daunting and data interpretation nontrivial. As the first of two back‐to‐back reviews on NMR spectroscopy aimed at non‐spectroscopists, the present review first provides an introduction to the basics of macromolecular NMR spectroscopy, including a discussion of typical sample requirements and what information can be obtained from simple NMR experiments. We then review the use of NMR spectroscopy for determining the 3D structures of macromolecules and examine how to judge the quality of NMR‐derived structures.
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Cites
Protein backbone angle restraints from searching a database for chemical shift and sequence homology
Cornilescu, G., Delaglio, F., & Bax, A. (1999) · Journal of Biomolecular NMR · doi:10.1023/a:1008392405740 · Cited by 2,957
Cited by
Duan, L., Liu, X., & Zhang, J. Z. H. (2016) · Journal of the American Chemical Society · doi:10.1021/jacs.6b02682 · Cited by 465
An Introduction to B-Cell Epitope Mapping and In Silico Epitope Prediction
Potocnakova, L., Bhide, M., & Pulzova, L. B. (2016) · Journal of Immunology Research · doi:10.1155/2016/6760830 · Cited by 367
Nikolaev, Y., Ripin, N., Soste, M., Picotti, P., Iber, D., & Allain, F. H.-T. (2019) · Nature Methods · doi:10.1038/s41592-019-0495-7 · Cited by 20
Metadata
- Authors
- Kwan, A. H., Mobli, M., Gooley, P. R., King, G. F., & Mackay, J. P.
- Venue
- The FEBS Journal
- Publisher
- Wiley
- Published
- 2011-01-28
- Volume
- 278
- Issue
- 5
- Pages
- 687-703
- ISSN
- 1742-464X (ISSN-L)
- 1742-4658
- OpenAlex
- W2144376263
- Cited by
- 175
- References
- 1
- FWCI
- 7.66
- Type
- journal article
- Language
- en
- Open access
- closed
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- Kwan, A. H., Mobli, M., Gooley, P. R., King, G. F., & Mackay, J. P. (2011). Macromolecular NMR spectroscopy for the non‐spectroscopist. The FEBS Journal. https://doi.org/10.1111/j.1742-4658.2011.08004.x
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