Etanercept decreases tumor necrosis factor‐α activity in chronic wound fluid
Abstract
High levels of tumor necrosis factor‐α (TNF‐α), a pro‐inflammatory cytokine, are present in the wound fluid of chronic nonhealing wounds. This leads to increased inflammation, cytokine expression, and ultimately results in impaired wound healing and tissue destruction. Etanercept is a recombinant fusion protein that consists of the soluble TNF receptor (p75) linked to the Fc portion of human IgG1. It is an effective inhibitor of TNF‐α and has been shown to provide rapid and sustained improvement in rheumatoid arthritis by acting as a soluble receptor binding TNF‐α and preventing its proinflammatory activities. Therefore, the aim of this study was to determine whether Etanercept could inhibit TNF‐α activity in chronic wound fluid. Wound fluid was collected from the venous leg ulcers of 16 different patients. The effect of Etanercept on TNF‐α activity was evaluated using both a TNF‐α bioassay and an enzyme‐linked immunoassay. Etanercept was found to reduce the cytotoxic effect of chronic wound fluid on L929 fibroblasts by approximately 30% and neutralized TNF‐α binding in the enzyme‐linked immunoassay by up to 80%. Direct application of Etanercept to chronic wounds may therefore reduce the inflammatory activity of TNF‐α, which could reduce the chronicity of venous leg ulcers and thus aid in the healing of these wounds.
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High levels of TNFα are present in the wound fluid of chronic nonhealing wounds, leading to excessive inflammation ultimately resulting in impaired wound healing and tissue destruction.
“High levels of TNFα are present in the wound fluid of chronic nonhealing wounds, leading to excessive inflammation ultimately resulting in impaired wound healing and tissue destruction.63”
From The Innate Immune System in Acute and Chronic Wounds (2016) · cites this paper as [63]
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- Authors
- Cowin, A. J., Hatzirodos, N., Rigden, J., Fitridge, R., & Belford, D. A.
- Affiliations
- Women's and Children's Hospital (AU)
- Queen Elizabeth Hospital (AU)
- Corresponding author
- Cowin, Allison J.
- Venue
- Wound Repair and Regeneration
- Publisher
- Wiley
- Published
- 2006-07
- Volume
- 14
- Issue
- 4
- Pages
- 421-426
- ISSN
- 1067-1927 (ISSN-L)
- 1524-475X
- OpenAlex
- W2080890577
- Cited by
- 43
- References
- 22
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 1 · 2022: 1 · 2021: 4 · 2020: 3 · 2017: 1 · 2016: 1 · 2015: 11 · 2014: 2 · 2012: 2
- FWCI
- 1.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Wound Repair and Regeneration (publishedVersion)
- PubMed (publishedVersion)
- Adelaide Research & Scholarship (AR&S) (University of Adelaide) (submittedVersion)
- Topics
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Health Sciences › Medicine › Rheumatology › Rheumatoid Arthritis Research and Therapies
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and biological activity of medicinal plants
- Keywords
- etanercept, TNF-α, chronic wounds, wound fluid, venous leg ulcers, anti-TNF therapy, soluble TNF receptors, inflammation, wound healing, L929 fibroblasts, cytotoxicity
- MeSH terms
- Etanercept, Aged, Aged, 80 and over, Biological Assay, Chronic Disease, Enzyme-Linked Immunosorbent Assay, Fibroblasts, Humans, Immunoglobulin G, Immunologic Factors, Middle Aged, Tumor Necrosis Factor-alpha, Varicose Ulcer, Receptors, Tumor Necrosis Factor, Cell Culture Techniques
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