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Fourier Transform Infrared Spectroscopic Analysis of Protein Secondary Structures
Abstract
Infrared spectroscopy is one of the oldest and well established experimental techniques for the analysis of secondary structure of polypeptides and proteins. It is convenient, non-destructive, requires less sample preparation, and can be used under a wide variety of conditions. This review introduces the recent developments in Fourier transform infrared (FTIR) spectroscopy technique and its applications to protein structural studies. The experimental skills, data analysis, and correlations between the FTIR spectroscopic bands and protein secondary structure components are discussed. The applications of FTIR to the secondary structure analysis, conformational changes, structural dynamics and stability studies of proteins are also discussed.
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Cites
Protein secondary structures in water from second-derivative amide I infrared spectra
Dong, A., Huang, P., & Caughey, W. S. (1990) · Biochemistry · doi:10.1021/bi00465a022 · Cited by 1,333
Cited by
Abidi, N., Cabrales, L., & Haigler, C. H. (2014) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2013.01.074 · Cited by 658
FTIR analysis of natural and synthetic collagen
Riaz, T., Zeeshan, R., Zarif, F., Ilyas, K., Muhammad, N., Safi, S. Z., Rahim, A., Rizvi, S. A. A., & Rehman, I. U. (2018) · Applied Spectroscopy Reviews · doi:10.1080/05704928.2018.1426595 · Cited by 628
Tao, K., Levin, A., Adler-Abramovich, L., & Gazit, E. (2016) · Chemical Society Reviews · doi:10.1039/c5cs00889a · Cited by 482
ThT 101: a primer on the use of thioflavin T to investigate amyloid formation
Gade Malmos, K., Blancas-Mejia, L. M., Weber, B., Buchner, J., Ramirez-Alvarado, M., Naiki, H., & Otzen, D. (2017) · Amyloid · doi:10.1080/13506129.2017.1304905 · Cited by 445
Wang, K.-Q., Luo, S.-Z., Zhong, X.-Y., Cai, J., Jiang, S.-T., & Zheng, Z. (2017) · Food Chemistry · doi:10.1016/j.foodchem.2016.07.037 · Cited by 423
Metadata
- Authors
- Kong, J., & Yu, S.
- Venue
- Acta Biochimica et Biophysica Sinica
- Published
- 2007-08-01
- Volume
- 39
- Issue
- 8
- Pages
- 549-559
- ISSN
- 1672-9145 (ISSN-L)
- OpenAlex
- W2107735396
- Cited by
- 3,536
- References
- 1
- FWCI
- 7.25
- Type
- journal article
- Language
- en
- Open access
- closed
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- Kong, J., & Yu, S. (2007). Fourier Transform Infrared Spectroscopic Analysis of Protein Secondary Structures. Acta Biochimica et Biophysica Sinica. https://doi.org/10.1111/j.1745-7270.2007.00320.x
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