The DOI matches its Crossref record; 109 of the 109 references it cites were found in a registry or an index.
ThT 101: a primer on the use of thioflavin T to investigate amyloid formation
Open accessAbstract
Thioflavin T (ThT) has been widely used to investigate amyloid formation since 1989. While concerns have recently been raised about its use as a probe specific for amyloid, ThT still continues to be a very valuable tool for studying kinetic aspects of fibrillation and associated inhibition mechanisms. This review aims to provide a conceptual instruction manual, covering appropriate considerations and pitfalls related to the use of ThT. We start by giving a brief introduction to amyloid formation with focus on the morphology of different aggregate species, followed by a discussion of the quality of protein needed to obtain reliable fibrillation data. After an overview of the photochemical basis for ThT's amyloid binding properties and artifacts that may arise from this, we describe how to plan and analyze ThT assays. We conclude with recommendations for complementary techniques to address shortcomings in the ThT assay.
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Cites
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Cited by
Zhao, X., Li, L., Zhao, Y., An, H., Cai, Q., Lang, J., Han, X., Peng, B., Fei, Y., Liu, H., et al. (2019) · Angewandte Chemie International Edition · doi:10.1002/anie.201908185 · Cited by 147
Medical Plants and Nutraceuticals for Amyloid-β Fibrillation Inhibition
Witter, S., Witter, R., Vilu, R., & Samoson, A. (2018) · Journal of Alzheimer s Disease Reports · doi:10.3233/adr-180066 · Cited by 25
Intramolecular interaction kinetically regulates fibril formation by human and mouse α-synuclein
Ohgita, T., Kono, H., Morita, I., Oyama, H., Shimanouchi, T., Kobayashi, N., & Saito, H. (2023) · Scientific Reports · doi:10.1038/s41598-023-38070-4 · Cited by 20
Atomic-level differences between brain parenchymal- and cerebrovascular-seeded Aβ fibrils
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Jami, K. M., Corbett, K. E., Farb, D. C., Osumi, K. M., Shafer, C. C., Criscione, S., & Murray, D. T. (2026) · Biophysical Chemistry · doi:10.1016/j.bpc.2026.107590 · Cited by 2
Metadata
- Authors
- Gade Malmos, K., Blancas-Mejia, L. M., Weber, B., Buchner, J., Ramirez-Alvarado, M., Naiki, H., & Otzen, D.
- Venue
- Amyloid
- Publisher
- Informa UK Limited
- Published
- 2017-01-02
- Volume
- 24
- Issue
- 1
- Pages
- 1-16
- ISSN
- 1350-6129 (ISSN-L)
- 1744-2818
- OpenAlex
- W2606007784
- Cited by
- 445
- References
- 109
- FWCI
- 14.76
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Gade Malmos, K., Blancas-Mejia, L. M., Weber, B., Buchner, J., Ramirez-Alvarado, M., Naiki, H., & Otzen, D. (2017). ThT 101: a primer on the use of thioflavin T to investigate amyloid formation. Amyloid. https://doi.org/10.1080/13506129.2017.1304905
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