The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
INACTIVATION OF GRAM‐NEGATIVE BACTERIA BY PHOTOSENSITIZED PORPHYRINS
Abstract
Abstract— Photosensitization of Escherichia coli and Pseudomonas aeruginosa cells by deuteroporphyrin (DP) is shown to be possible in the presence of the polycationic agent polymyxin nonapeptide (PMNP). Previous studies established complete resistance of Gram‐negative bacteria to the photodynamic effects of porphyrins. The present results show that combined treatment of E. coli or P. aeruginosa cultures with DP and PMNP inhibit cell growth and viability. No antibacterial activity of PMNP alone could be demonstrated and cell viability remained unchanged. Spectroscopically, PMNP was found to bind DP, a mechanism which probably assists its penetration into the cell's membranes. Insertion of DP into the cells was monitored by the characteristic fluorescence band of bound DP at 622 nm. Binding times were 5–40 min and the extent of binding increased with decreasing the pH from 8.5 to 6.5. DP binding constants, as well as the concentrations of PMNP which were required for maximal effect on the various Gram‐negative bacteria, were determined fluorometrically. By the treatment of DP, PMNP and light the growth of E. coli and P. aeruginosa cultures was stopped and the viability of the culture was dramatically reduced. Within 60 min of treatment the survival fraction of E. coli culture was 9 × 10–6 that of P. aeruginosa was 5.2 × 10–4. Electron microscopy depicted ultrastructural alterations in the Gram‐negative cells treated by DP and PMNP. The completion of cell division was inhibited and the chromosomal domain was altered markedly.
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Cites
Malik, Z., Hanania, J., & Nitzan, Y. (1990) · Journal of Photochemistry and Photobiology B: Biology · doi:10.1016/1011-1344(90)85044-w · Cited by 316
Self-citationPolycations as outer membrane-disorganizing agents
Vaara, M., & Vaara, T. (1983) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.24.1.114 · Cited by 250
Polycations sensitize enteric bacteria to antibiotics
Vaara, M., & Vaara, T. (1983) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.24.1.107 · Cited by 214
The Behavior of Oxonol Dyes in Phospholipid Dispersions
Bashford, C. L., Chance, B., Smith, J. C., & Yoshida, T. (1979) · Biophysical Journal · doi:10.1016/s0006-3495(79)85278-9 · Cited by 113
HEMATOPORPHYRIN‐SENSITIZED PHOTOINACTIVATION OF Streptococcus faecalis
Bertoloni, G., Salvato, B., Dall‘Acqua, M., Vazzoler, M., & Jori, G. (1984) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1984.tb08864.x · Cited by 111
Ehrenberg, B., Malik, Z., & Nitzan, Y. (1985) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1985.tb03508.x · Cited by 95
Self-citationEffect of photoactivated hematoporphyrin derivative on the viability ofStaphylococcus aureus
Nitzan, Y., Gozhansky, S., & Malik, Z. (1983) · Current Microbiology · doi:10.1007/bf01577728 · Cited by 73
Self-citationTHE PHOTODYNAMIC EFFECT OF HEMATOPORPHYRIN ON DNA
Boye, E., & Moan, J. E. (1980) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1980.tb03711.x · Cited by 68
Bactericidal Effects of Photoradiation Therapy With Hematoporphyrin Derivative
Venezio, F. R., DiVincenzo, C. A., Sherman, R., Reichman, M. E., Origitano, T. C. O., Thompson, K. D., & Reichman, O. H. (1985) · The Journal of Infectious Diseases · doi:10.1093/infdis/151.1.166 · Cited by 65
Photodynamic effects of deuteroporphyrin on Gram-positive bacteria
Nitzan, Y., Shainberg, B., & Malik, Z. (1987) · Current Microbiology · doi:10.1007/bf01589376 · Cited by 54
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Cited by
Antimicrobial Photodynamic Therapy to Kill Gram-negative Bacteria
Sperandio, F. F., Huang, Y.-Y., & Hamblin, M. R. (2014) · Recent Patents on Anti-Infective Drug Discovery · doi:10.2174/1574891x113089990012 · Cited by 585
The role of singlet oxygen and oxygen concentration in photodynamic inactivation of bacteria
Maisch, T., Baier, J., Franz, B., Maier, M., Landthaler, M., Szeimies, R.-M., & Bäumler, W. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0611328104 · Cited by 428
Photodynamic Therapy Targeted to Pathogens
Demidova, T. N., & Hamblin, M. R. (2004) · International Journal of Immunopathology and Pharmacology · doi:10.1177/039463200401700304 · Cited by 356
Anti-microbial photodynamic therapy: useful in the future?
Maisch, T. (2007) · Lasers in Medical Science · doi:10.1007/s10103-006-0409-7 · Cited by 356
Photodynamic therapy in the control of oral biofilms
Soukos, N. S., & Goodson, J. M. (2011) · Periodontology 2000 · doi:10.1111/j.1600-0757.2010.00346.x · Cited by 342
Metadata
- Authors
- Nitzan, Y., Gutterman, M., Malik, Z., & Ehrenberg, B.
- Venue
- Photochemistry and Photobiology
- Publisher
- Wiley
- Published
- 1992-01
- Volume
- 55
- Issue
- 1
- Pages
- 89-96
- ISSN
- 0031-8655 (ISSN-L)
- 1751-1097
- OpenAlex
- W1986057738
- Cited by
- 340
- References
- 15
- FWCI
- 3.91
- Type
- journal article
- Language
- en
- Open access
- closed
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- Nitzan, Y., Gutterman, M., Malik, Z., & Ehrenberg, B. (1992). INACTIVATION OF GRAM‐NEGATIVE BACTERIA BY PHOTOSENSITIZED PORPHYRINS. Photochemistry and Photobiology. https://doi.org/10.1111/j.1751-1097.1992.tb04213.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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