The DOI matches its Crossref record; 103 of the 107 references it cites were found in a registry or an index.
Microbial degradation of lignin: how a bulky recalcitrant polymer is efficiently recycled in nature and how we can take advantage of this
Open accessAbstract
Summary: Lignin is the second most abundant constituent of the cell wall of vascular plants, where it protects cellulose towards hydrolytic attack by saprophytic and pathogenic microbes. Its removal represents a key step for carbon recycling in land ecosystems, as well as a central issue for industrial utilization of plant biomass. The lignin polymer is highly recalcitrant towards chemical and biological degradation due to its molecular architecture, where different non‐phenolic phenylpropanoid units form a complex three‐dimensional network linked by a variety of ether and carbon–carbon bonds. Ligninolytic microbes have developed a unique strategy to handle lignin degradation based on unspecific one‐electron oxidation of the benzenic rings in the different lignin substructures by extracellular haemperoxidases acting synergistically with peroxide‐generating oxidases. These peroxidases posses two outstanding characteristics: (i) they have unusually high redox potential due to haem pocket architecture that enables oxidation of non‐phenolic aromatic rings, and (ii) they are able to generate a protein oxidizer by electron transfer to the haem cofactor forming a catalytic tryptophanyl‐free radical at the protein surface, where it can interact with the bulky lignin polymer. The structure–function information currently available is being used to build tailor‐made peroxidases and other oxidoreductases as industrial biocatalysts.
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“These C sources can contain complex plant-based OM which is considered rather resistant to chemical and biological degradation 26,27 as well as labile low-molecular-weight C such as amino acids, carbohydrates and carboxylic acids that can be easily used by microorganisms to fuel microbially mediated Fe(III) reduction leading to As release 16,28,29 .”
From Role of in Situ Natural Organic Matter in Mobilizing As during Microbial Reduction of FeIII-Mineral-Bearing Aquifer Sediments from Hanoi (Vietnam). (2020) · cites this paper as [26]
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“For PS degradation, enzymes similar to those utilised by ligninoxidising microbes were suggested, 114,118”
From The fate of plastic in the ocean environment – a minireview (2021) · cites this paper as [118]
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Cites
Enzymatic "Combustion": The Microbial Degradation Of Lignin
Kirk, T. K., & Farrell, R. L. (1987) · Annual Review of Microbiology · doi:10.1146/annurev.mi.41.100187.002341 · Cited by 2,627
Fungal laccases – occurrence and properties
Baldrián, P. (2006) · FEMS Microbiology Reviews · doi:10.1111/j.1574-4976.2005.00010.x · Cited by 2,251
Polymer blends and composites from renewable resources
Yu, L., Dean, K., & Li, L. (2006) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2006.03.002 · Cited by 2,107
Lignin chemistry?past, present and future
Adler, E. (1977) · Wood Science and Technology · doi:10.1007/bf00365615 · Cited by 1,825
Lignins: Natural polymers from oxidative coupling of 4-hydroxyphenyl- propanoids
Ralph, J., Lundquist, K., Brunow, G., Lu, F., Kim, H., Schatz, P. F., Marita, J. M., Hatfield, R. D., Ralph, S. A., Christensen, J. H., et al. (2004) · Phytochemistry Reviews · doi:10.1023/b:phyt.0000047809.65444.a4 · Cited by 1,666
Microbial and Enzymatic Degradation of Wood and Wood Components
Eriksson, K. L., Blanchette, R. A., & Ander, P. (1990) · Springer series in wood science · doi:10.1007/978-3-642-46687-8 · Cited by 1,408
Oxidation of non‐phenolic substrates
Bourbonnais, R., & Paice, M. G. (1990) · FEBS Letters · doi:10.1016/0014-5793(90)80298-w · Cited by 1,346
Laccases: blue enzymes for green chemistry
Riva, S. (2006) · Trends in biotechnology · doi:10.1016/j.tibtech.2006.03.006 · Cited by 1,245
Lignin-Degrading Enzyme from the Hymenomycete Phanerochaete chrysosporium Burds
Tien, M., & Kirk, T. K. (1983) · Science · doi:10.1126/science.221.4611.661 · Cited by 1,211
Martı́nez, Á. T., Speranza, M., Ruiz-Dueñas, F. J., Ferreira, P., Camarero, S., Guillén, F., Martı́nez, M. J., Gutiérrez, A., & del Río, J. C. (2005) · PubMed · Cited by 1,064
Self-citation
Research works printed but not linked: 5
- Dunford H.B., 1999, Heme Peroxidases
- 10.1021/es062723 · DOI
- Marques G. Gamelas J.A.F. Evtuguin D. Ruiz‐Dueñas F.J. Morales M. Del Río J.C. et al.(2008)Versatile peroxidase reoxidation of Mn‐based polyoxomethalate in chlorine‐free bleaching of eucalypt pulp.Proceeding of the 8th International Peroxidase Symposium Tampere 20–23 August.
- Paice M.G., 1995, Oxidative bleaching enzymes: a review, J Pulp Paper Sci, 21, J280
- Timofeevski S.L., 1999, Engineering a functional hybrid of manganese peroxidase and lignin peroxidase, Plant Peroxidase Newsl, 13, 99
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Sorted by citations
Expansion of the enzymatic repertoire of the CAZy database to integrate auxiliary redox enzymes
Levasseur, A., Drula, É., Lombard, V., Coutinho, P. M., & Henrissat, B. (2013) · Biotechnology for Biofuels · doi:10.1186/1754-6834-6-41 · Cited by 1,373
Shi, Y., Chai, L., Tang, C., Yang, Z., Zhang, H., Chen, R., Chen, Y., & Yu, Z. (2013) · Biotechnology for Biofuels · doi:10.1186/1754-6834-6-1 · Cited by 1,232
Catalytic fast pyrolysis of lignocellulosic biomass
Liu, C., Wang, H., Karim, A. M., Sun, J., & Wang, Y. (2014) · Chemical Society Reviews · doi:10.1039/c3cs60414d · Cited by 1,131
Laccase Properties, Physiological Functions, and Evolution
Janusz, G., Pawlik, A., Świderska-Burek, U., Polak, J., Sulej, J., Jarosz‐Wilkołazka, A., & Paszczynski, A. J. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21030966 · Cited by 756
Wang, L., O’Connor, D., Rinklebe, J., Ok, Y. S., Tsang, D. C. W., Shen, Z., & Hou, D. (2020) · Environmental Science & Technology · doi:10.1021/acs.est.0c04033 · Cited by 655
Metadata
- Authors
- Ruiz‐Dueñas, F. J., & Martínez, Á. T.
- Venue
- Microbial Biotechnology
- Publisher
- Wiley
- Published
- 2009-02-18
- Volume
- 2
- Issue
- 2
- Pages
- 164-177
- ISSN
- 1751-7915 (ISSN-L)
- PMC
- PMC3815837
- OpenAlex
- W2129126200
- Cited by
- 561
- References
- 107
- Citations by year
- 2026: 26 · 2025: 31 · 2024: 29 · 2023: 37 · 2022: 27 · 2021: 40 · 2020: 47 · 2019: 32 · 2018: 45 · 2017: 42 · 2016: 48 · 2015: 33 · 2014: 38 · 2013: 30 · 2012: 22
- FWCI
- 1.61
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Microbial Biotechnology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- Funders
- Universidad de Alcalá
- Topics
- Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Enzyme-mediated dye degradation
- Physical Sciences › Chemical Engineering › Process Chemistry and Technology › Lignin and Wood Chemistry
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Keywords
- lignin degradation, ligninolytic microorganisms, heme peroxidase, lignin recalcitrance, lignin substructures, plant biomass utilization, carbon recycling, industrial biocatalysts, ligninolytic enzymes
- MeSH terms
- Biodegradation, Environmental, Fungal Proteins, Fungi, Lignin, Biocatalysis, Recycling
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