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Cited by 112Essential Oils and Antimicrobial ActivityBacterial Identification and Susceptibility TestingAntimicrobial agents and applications

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The agar microdilution method - a new method for antimicrobial susceptibility testing for essential oils and plant extracts

Golus, J., Sawicki, R., Widelski, J., Ginalska, G.·2016·Journal of Applied Microbiology
DOI 10.1111/jam.13253
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Abstract

Aims To develop a new agar microdilution technique suitable for the assessment of the antimicrobial activity of natural plant products such as essential oils or plant extracts as well as to evaluate the antimicrobial effect of several essential oils and plant extracts. Methods and results The proposed agar microdilution method was evolved on the basis of the CLSI agar dilution method, approved for conventional antimicrobials. However, this new method combines convenience and time/cost effectiveness typical for microtitre methods with the advantages of the agar dilution of hydrophobic or coloured substances. Different concentrations of the tested agents were added to Eppendorf tubes with molten Mueller-Hinton agar, vortexed and dispensed into the 96-well microplate in a small volume of 100 μl per well which allows for rapid, easy and economical preparation of samples as well as providing a uniform and stable dispersion without separation of the oil-water phases which occurs in methods with liquid medium. Next, the agar microdilution plates were inoculated with four reference bacterial strains. The results of our study demonstrated that the minimal inhibitory concentrations (MICs) were successfully determined using the agar microdilution method even with hydrophobic essential oils or strongly coloured plant extracts. Conclusions The new agar microdilution method avoids the problems associated with testing of water insoluble, oily or strongly coloured plant natural products. Mor…

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Metadata

Authors
Golus, J., Sawicki, R., Widelski, J., & Ginalska, G.
Affiliations
Medical University of Lublin (PL)
Corresponding author
Golus, J.
ORCID
Venue
Journal of Applied Microbiology
Publisher
Oxford University Press (OUP)
Published
2016-10-19
Volume
121
Issue
5
Pages
1291-1299
ISSN
1364-5072 (ISSN-L)
DOI
10.1111/jam.13253
OpenAlex
W2513472555
Cited by
112
References
2
Citations by year
2026: 8 · 2025: 30 · 2024: 15 · 2023: 12 · 2022: 14 · 2021: 13 · 2020: 11 · 2019: 6 · 2018: 1 · 2017: 2
FWCI
3.77
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Journal of Applied Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Ministerstwo Nauki i Szkolnictwa Wyższego
  • Medical University of Lublin
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
  • Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Antimicrobial agents and applications
Keywords
antimicrobial susceptibility testing, essential oils, plant extracts, minimum inhibitory concentration, hydrophobic compounds, 96-well plate, antibacterial activity, plant natural products
MeSH terms
Agar, Anti-Bacterial Agents, Microbial Sensitivity Tests, Oils, Volatile, Plant Extracts, Drug Resistance, Bacterial

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CoChat. (2026). Citation check: The agar microdilution method - a new method for antimicrobial susceptibility testing for essential oils and plant extracts. https://app.cochat.ai/papers/10.1111/jam.13253
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Golus, J., Sawicki, R., Widelski, J., & Ginalska, G. (2016). The agar microdilution method - a new method for antimicrobial susceptibility testing for essential oils and plant extracts. Journal of Applied Microbiology. https://doi.org/10.1111/jam.13253