Cited by 27Cardiac electrophysiology and arrhythmiasCongenital heart defects researchCardiac Arrhythmias and Treatments

The sinus venosus myocardium contributes to the atrioventricular canal: potential role during atrioventricular node development?

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Kelder, Tim P., Vicente‐Steijn, Rebecca, Harryvan, Tom J., Kosmidis, Georgios, Gittenberger‐de Groot, Adriana C., Poelmann, Rob E., Schalij, Martin J., DeRuiter, Marco C. +1 more·2015·Journal of Cellular and Molecular Medicine

Abstract

The presence of distinct electrophysiological pathways within the atrioventricular node (AVN) is a prerequisite for atrioventricular nodal reentrant tachycardia to occur. In this study, the different cell contributions that may account for the anatomical and functional heterogeneity of the AVN were investigated. To study the temporal development of the AVN, the expression pattern of ISL1, expressed in cardiac progenitor cells, was studied in sequential stages performing co-staining with myocardial markers (TNNI2 and NKX2-5) and HCN4 (cardiac conduction system marker). An ISL1+/TNNI2+/HCN4+ continuity between the myocardium of the sinus venosus and atrioventricular canal was identified in the region of the putative AVN, which showed a pacemaker-like phenotype based on single cell patch-clamp experiments. Furthermore, qPCR analysis showed that even during early development, different cell populations can be identified in the region of the putative AVN. Fate mapping was performed by in ovo vital dye microinjection. Embryos were harvested and analysed 24 and 48 hrs post-injection. These experiments showed incorporation of sinus venosus myocardium in the posterior region of the atrioventricular canal. The myocardium of the sinus venosus contributes to the atrioventricular canal. It is postulated that the myocardium of the sinus venosus contributes to nodal extensions or transitional cells of the AVN since these cells are located in the posterior region of the AVN. This finding may help to understand the origin of atrioventricular nodal reentrant tachycardia.

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Authors
Kelder, T. P., Vicente‐Steijn, R., Harryvan, T. J., Kosmidis, G., Gittenberger‐de Groot, A. C., Poelmann, R. E., Schalij, M. J., DeRuiter, M. C., & Jongbloed, M. R. M.
Affiliations
  • Leiden University Medical Center (NL)
  • Department of Anatomy & Embryology Leiden University Medical Center Leiden The Netherlands
Corresponding author
Jongbloed, Monique R.M.
Venue
Journal of Cellular and Molecular Medicine
Publisher
Wiley
Published
2015-03-06
Volume
19
Issue
6
Pages
1375-1389
ISSN
  • 1582-1838 (ISSN-L)
  • 1582-4934
OpenAlex
W2036410326
Cited by
27
References
38
Citations by year
2026: 1 · 2024: 3 · 2021: 4 · 2020: 1 · 2019: 5 · 2018: 6 · 2017: 3 · 2016: 3 · 2015: 1
FWCI
3.12
Type
journal article
Language
en
Open access
Locations
  • Journal of Cellular and Molecular Medicine (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
  • Leiden Repository (Leiden University) (submittedVersion)
Funders
ZonMw/NWO (90700368)
Topics
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Arrhythmias and Treatments
Keywords
atrioventricular node, atrioventricular canal, atrioventricular nodal reentrant tachycardia, Isl1, HCN4, TNNI2, Nkx2.5, cardiac pacemaker, fate mapping, embryonic heart development, cardiac conduction system
MeSH terms
Animals, Atrioventricular Node, Chick Embryo, Heart, Immunohistochemistry, Membrane Potentials, Microscopy, Fluorescence, Myocardium, In Situ Hybridization, Homeodomain Proteins, Patch-Clamp Techniques, Gene Expression Regulation, Developmental, Troponin I, Reverse Transcriptase Polymerase Chain Reaction, Imaging, Three-Dimensional, Avian Proteins, Myocytes, Cardiac, LIM-Homeodomain Proteins, Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels

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