Antagonists of Wnt/β-catenin signalling in the periodontitis associated with type 2 diabetes and smoking
Abstract
Aim: This study evaluated the levels of sclerostin (SOST) and Dickkopf (DKK)‐1 in the chronic periodontitis (CP) associated with type 2 diabetes (DM) and/or smoking. Relationships between SOST, DDK 1, RANKL, OPG, IL‐1ß, IL‐6 and TNF‐α, and pathogens were assessed. Material and Methods: The study population included non‐diabetic non‐smokers (control), non‐smokers with DM (DM group), non‐diabetic smokers (S group) and smokers with DM (SDM group), all with CP. Serum and gingival levels of SOST, DKK 1, RANKL, OPG, IL‐1ß, IL‐6 and TNF‐α were evaluated by multiplex immunoassay. Gene expressions of these biomarkers and subgingival levels of pathogens were assessed by qPCR. Results: Gingival protein and/or mRNA levels of DKK 1 and SOST were higher in subjects with DM and/or smoking than in controls (p < .05). Serum levels of SOST were higher in the DM group than in controls (p < .05). DKK 1 positively correlated with SOST in the DM, SDM and control groups (p < .05) at mRNA levels. DKK‐1 and SOST correlated with pathogens, especially in both groups with DM. Conclusions: SOST and DKK 1 were upregulated in patients with CP presenting DM and/or smoking. DM, alone or with smoking, particularly influenced the correlations of SOST and DKK 1 with each other and with the other biomarkers mostly at mRNA levels, as well as with periodontal pathogens.
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Smoking could enhance the levels of DKK1 and sclerostin in patients with periodontitis.
“Miranda et al. revealed that smoking could enhance the levels of DKK1 and sclerostin in patients with periodontitis [107].”
From An Overlooked Bone Metabolic Disorder: Cigarette Smoking-Induced Osteoporosis (2022) · cites this paper as [107]
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An Overlooked Bone Metabolic Disorder: Cigarette Smoking-Induced Osteoporosis
Weng, W., Li, H., & Zhu, S. (2022) · Genes · doi:10.3390/genes13050806 · Cited by 65 · see citing works
- Authors
- Miranda, T. S., Napimoga, M. H., Feres, M., Marins, L. M., da Cruz, D. F., da Silva, H. D. P., & Duarte, P. M.
- Affiliations
- Guarulhos University (BR)
- Faculdade São Leopoldo Mandic (BR)
- Dental Research Division Department of Periodontology Guarulhos University São Paulo Brazil
- Corresponding author
- Duarte, Poliana M.
- Venue
- Journal of Clinical Periodontology
- Publisher
- Wiley
- Published
- 2018-01-12
- Volume
- 45
- Issue
- 3
- Pages
- 293-302
- ISSN
- 0303-6979 (ISSN-L)
- 1600-051X
- OpenAlex
- W2773662766
- Cited by
- 41
- References
- 43
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 4 · 2023: 11 · 2022: 6 · 2021: 2 · 2020: 4 · 2019: 4 · 2018: 1
- FWCI
- 3.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal Of Clinical Periodontology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo (São Paulo, São Paulo, Brazil, # 2013/23743‐9; 2013/10354‐4)
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasília, Distrito Federal, Brasil, 441367/2014‐3)
- Topics
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Keywords
- chronic periodontitis, type 2 diabetes, smoking, sclerostin, DKK1, Wnt/β-catenin signaling, RANKL, osteoprotegerin, IL-1β, interleukin-6, TNF-α, periodontal pathogens, serum biomarkers
- MeSH terms
- Adult, Aged, Analysis of Variance, Diabetes Mellitus, Type 2, Female, Genetic Markers, Humans, Male, Middle Aged, RNA, Messenger, Smoking, Biomarkers, Up-Regulation, Linear Models, Case-Control Studies, Bone Morphogenetic Proteins, Intercellular Signaling Peptides and Proteins, Adaptor Proteins, Signal Transducing, Wnt Proteins, beta Catenin, Chronic Periodontitis, Wnt Signaling Pathway
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