Cited by 41Oral microbiology and periodontitis researchWnt/β-catenin signaling in development and cancerBone Metabolism and Diseases

Antagonists of Wnt/β-catenin signalling in the periodontitis associated with type 2 diabetes and smoking

Miranda, Tamires S., Napimoga, Marcelo H., Feres, Magda, Marins, Letícia M., da Cruz, Daniele F., da Silva, Hélio Doyle P., Duarte, Poliana M.·2018·Journal of Clinical Periodontology

Abstract

Aim: This study evaluated the levels of sclerostin (SOST) and Dickkopf (DKK)‐1 in the chronic periodontitis (CP) associated with type 2 diabetes (DM) and/or smoking. Relationships between SOST, DDK 1, RANKL, OPG, IL‐1ß, IL‐6 and TNF‐α, and pathogens were assessed. Material and Methods: The study population included non‐diabetic non‐smokers (control), non‐smokers with DM (DM group), non‐diabetic smokers (S group) and smokers with DM (SDM group), all with CP. Serum and gingival levels of SOST, DKK 1, RANKL, OPG, IL‐1ß, IL‐6 and TNF‐α were evaluated by multiplex immunoassay. Gene expressions of these biomarkers and subgingival levels of pathogens were assessed by qPCR. Results: Gingival protein and/or mRNA levels of DKK 1 and SOST were higher in subjects with DM and/or smoking than in controls (p < .05). Serum levels of SOST were higher in the DM group than in controls (p < .05). DKK 1 positively correlated with SOST in the DM, SDM and control groups (p < .05) at mRNA levels. DKK‐1 and SOST correlated with pathogens, especially in both groups with DM. Conclusions: SOST and DKK 1 were upregulated in patients with CP presenting DM and/or smoking. DM, alone or with smoking, particularly influenced the correlations of SOST and DKK 1 with each other and with the other biomarkers mostly at mRNA levels, as well as with periodontal pathogens.

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Crossref · 10.1111/jcpe.12854

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Authors
Miranda, T. S., Napimoga, M. H., Feres, M., Marins, L. M., da Cruz, D. F., da Silva, H. D. P., & Duarte, P. M.
Affiliations
  • Guarulhos University (BR)
  • Faculdade São Leopoldo Mandic (BR)
  • Dental Research Division Department of Periodontology Guarulhos University São Paulo Brazil
Corresponding author
Duarte, Poliana M.
Venue
Journal of Clinical Periodontology
Publisher
Wiley
Published
2018-01-12
Volume
45
Issue
3
Pages
293-302
ISSN
  • 0303-6979 (ISSN-L)
  • 1600-051X
OpenAlex
W2773662766
Cited by
41
References
43
Citations by year
2026: 5 · 2025: 4 · 2024: 4 · 2023: 11 · 2022: 6 · 2021: 2 · 2020: 4 · 2019: 4 · 2018: 1
FWCI
3.40
Type
journal article
Language
en
Open access
closed
Locations
  • Journal Of Clinical Periodontology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (São Paulo, São Paulo, Brazil, # 2013/23743‐9; 2013/10354‐4)
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasília, Distrito Federal, Brasil, 441367/2014‐3)
Topics
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
Keywords
chronic periodontitis, type 2 diabetes, smoking, sclerostin, DKK1, Wnt/β-catenin signaling, RANKL, osteoprotegerin, IL-1β, interleukin-6, TNF-α, periodontal pathogens, serum biomarkers
MeSH terms
Adult, Aged, Analysis of Variance, Diabetes Mellitus, Type 2, Female, Genetic Markers, Humans, Male, Middle Aged, RNA, Messenger, Smoking, Biomarkers, Up-Regulation, Linear Models, Case-Control Studies, Bone Morphogenetic Proteins, Intercellular Signaling Peptides and Proteins, Adaptor Proteins, Signal Transducing, Wnt Proteins, beta Catenin, Chronic Periodontitis, Wnt Signaling Pathway

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