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Cited by 15Natural Antidiabetic Agents Studiesmelanin and skin pigmentationAgricultural and Biological Research

Optimization of ultrasound‐assisted extraction of melanin and its hypoglycemic activities from Sporisorium reilianum

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Lu, Ming, Yu, Miao, Shi, Taiyuan, Ma, Jiahui, Fu, Xin, Meng, Xianjun, Shi, Lin·2020·Journal of Food Processing and Preservation
DOI 10.1111/jfpp.14707
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Abstract

Sporisorium reilianum (S. reiliana) is rich in nutrition, high in melanin content, and can be obtained by artificial species. It is a high-quality raw material for extracting natural melanin. Because of its unique structure and physicochemical property, natural melanin has become a stable and unique rare species in natural pigments. In this study, the effect of ultrasonic assisted on melanin extraction was studied with S. reiliana as a material. The influences of ultrasonic power, temperature, time, NaOH extract on melanin extraction in S. reiliana were investigated respectively. And through the single factor test and response surface method (RSM) to verify the evaluation. The RSM optimum conditions for melanin accumulation were: Ultrasonic power 245W, temperature 50°C, time 2 h, and pH 13 of NaOH extract. Under this condition, the return is 3.52%, which is close to the predicted value, indicating the feasibility of this model. For α-glycosidase and PTP1B inhibition assay, the melanin demonstrated the inhibitory effect with IC50 82.16 ± 5.02 μg/ml and 81.27 ± 10.48 μg/ml, respectively. This study reveals the hypoglycemic activities of the melanin isolated from S. reiliana for the first time. Practical applications Melanin from S. reiliana is an excellent hypoglycemic product due to its high α-glycosidase and PTP1B inhibition activity. However, its extraction yield is too low. The combination of unconventional techniques (ultrasound-assisted alkali dissolution and acid precipitation) is characterized as a new methodology for the extraction of melanin from S. reiliana. The extract provided a higher content of melanin than conventional methods. This technique is easily incorporated at the industrial level and is the low cost after the purchase of the relevant equipment.

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  • Fungi like Agaricus bisporus, A. niger, Sphacelotheca reiliana, and Dothistroma septosporum can synthesize natural DOPA melanin without exogenous substrate addition.

    “In contrast, fungi like Agaricus bisporus [22,23], A. niger and Sphacelotheca reiliana [24], and Dothistroma septosporum [25] can synthesize natural DOPA melanin without exogenous substrate addition.”

    From Fungal Melanin in Plant Pathogens: Complex Biosynthesis Pathways and Diverse Biological Functions (2025) · cites this paper as [24]

    The Abstract (page unavailable) identifies S. reiliana as a source of natural melanin, supporting that portion of the statement attached to [24]. It does not identify the pigment as DOPA melanin or establish synthesis without an exogenous substrate; searches for those terms and Aspergillus niger found no supporting passage about A. niger. (LLM, conf=0.86)

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Cited by

Sorted by citations

Authors
Lu, M., Yu, M., Shi, T., Ma, J., Fu, X., Meng, X., & Shi, L.
Affiliations
  • Shenyang Agricultural University (CN)
  • Liaoning Academy of Agricultural Sciences (CN)
  • Shenyang Normal University (CN)
Corresponding author
  • Meng, Xianjun
  • Shi, Lin
ORCID
Venue
Journal of Food Processing and Preservation
Publisher
Hindawi Limited
Published
2020-08-11
Volume
44
Issue
9
ISSN
  • 0145-8892 (ISSN-L)
  • 1745-4549
DOI
10.1111/jfpp.14707
OpenAlex
W3048633990
Cited by
15
References
0
Citations by year
2026: 3 · 2025: 4 · 2024: 1 · 2023: 5 · 2022: 2
FWCI
1.28
Type
journal article
Language
en
Open access
Licence
Locations
Journal of Food Processing and Preservation (publishedVersion)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Natural Antidiabetic Agents Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
Keywords
melanin, Sporisorium reilianum, ultrasound-assisted extraction, hypoglycemic activity, α-glycosidase inhibition, PTP1B inhibition, natural pigments, extraction optimization, alkaline extraction, acid rain, response surface methodology

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