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Cited by 153Essential Oils and Antimicrobial ActivityAntimicrobial agents and applicationsAntimicrobial Resistance in Staphylococcus

The DOI matches its Crossref record.

Antimicrobial activity and mechanism of limonene against Staphylococcus aureus

Han, Yingjie, Chen, Wenxue, Sun, Zhichang·2021·Journal of Food Safety
DOI 10.1111/jfs.12918
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Abstract

In this study, inhibitory effect of limonene on Staphylococcus aureus (S. aureus) and its antibacterial mechanism was explored, respectively. The results showed that limonene effectively inhibited bacterial growth at a minimum inhibitory concentration (MIC) of 20 ml/L. Scanning electron microscopy (SEM), the reduction of AKP activity and fluorescence microscope observation confirmed that limonene caused the destruction of the cell morphology and cell wall integrity of S. aureus. The reduction of MFI in fluorescein diacetate staining experiment and leakage of biological macromolecules (nucleic acids and proteins) indicated that limonene damaged the cell membrane and increased membrane permeability. Besides, the reduction of membrane potential (MP) further confirmed that the damage to membrane and the reduction of respiratory metabolic activity. Furthermore, the respiratory depression test confirmed that the pathway of limonene affecting the respiratory metabolism of S. aureus is TCA pathway, followed by EMP. In addition, the disorder of succinate dehydrogenase (SDH), malate dehydrogenase (MDH), pyruvate kinase (PK), ATPase (T‐ATPase, Na+‐K+‐ATPase, Ca 2+‐Mg 2+‐ATPase), and ATP concentration demonstrated that limonene could result in a slowdown of metabolism and metabolic dysfunction, thus inhibiting ATP synthesis.

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Metadata

Authors
Han, Y., Chen, W., & Sun, Z.
Affiliations
  • Hainan University (CN)
  • College of Food Sciences & Engineering, Hainan University Haikou China
ORCID
Venue
Journal of Food Safety
Publisher
Wiley
Published
2021-08-15
Volume
41
Issue
5
ISSN
  • 0149-6085 (ISSN-L)
  • 1745-4565
DOI
10.1111/jfs.12918
OpenAlex
W3194728966
Cited by
153
References
0
Citations by year
2026: 28 · 2025: 32 · 2024: 50 · 2023: 27 · 2022: 15 · 2021: 1
FWCI
13.89
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
Journal of Food Safety (publishedVersion)
Funders
National Natural Science Foundation of China (31640061, 31760480)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Antimicrobial agents and applications
  • Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
Keywords
limonene, Staphylococcus aureus, antibacterial activity, cell membrane damage, membrane permeability, membrane potential, cell wall integrity, cell morphology, TCA cycle, glycolysis, ATP synthesis inhibition, succinate dehydrogenase, pyruvate kinase, Na+/K+-ATPase, Ca2+-Mg2+-ATPase, minimum inhibitory concentration

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