PE6c greatly enhances prime editing in transgenic rice plants
Open accessAbstract
Prime editing is a versatile CRISPR/Cas-based precise genome-editing technique for crop breeding. Four new types of prime editors (PEs) named PE6a-d were recently generated using evolved and engineered reverse transcriptase (RT) variants from three different sources. In this study, we tested the editing efficiencies of four PE6 variants and two additional PE6 constructs with double-RT modules in transgenic rice (Oryza sativa) plants. PE6c, with an evolved and engineered RT variant from the yeast Tf1 retrotransposon, yielded the highest prime-editing efficiency. The average fold change in the editing efficiency of PE6c compared with PEmax exceeded 3.5 across 18 agronomically important target sites from 15 genes. We also demonstrated the feasibility of using two RT modules to improve prime-editing efficiency. Our results suggest that PE6c or its derivatives would be an excellent choice for prime editing in monocot plants. In addition, our findings have laid a foundation for prime-editing-based breeding of rice varieties with enhanced agronomically important traits.
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Recent studies have demonstrated a significant increase in PE efficiency, with variants like PE6c achieving over threefold increases in editing efficiency in rice.
“Recent studies have demonstrated a significant increase in PE efficiency, with variants like PE6c achieving over threefold increases in editing efficiency in rice (Cao et al., 2024).”
From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Cao et al., 2024
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- Authors
- Cao, Z., Sun, W., Qiao, D., Wang, J., Li, S., Liu, X., Xin, C., Lu, Y., Gul, S. L., Wang, X., & Chen, Q.
- Affiliations
- China Agricultural University (CN)
- State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences China Agricultural University Beijing 100193 China
- ORCID
- Venue
- Journal of Integrative Plant Biology
- Publisher
- Wiley
- Published
- 2024-07-09
- Volume
- 66
- Issue
- 9
- Pages
- 1864-1870
- ISSN
- 0577-7496 (ISSN-L)
- 1672-9072
- 1744-7909
- OpenAlex
- W4400447375
- Cited by
- 41
- References
- 23
- Citations by year
- 2026: 27 · 2025: 12 · 2024: 2
- FWCI
- 5.56
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Integrative Plant Biology (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Key Research and Development Program of China (2023YFD1202905)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Keywords
- prime editing, CRISPR/Cas genome editing, transgenic rice, rice, editing efficiency, crop breeding
- MeSH terms
- Gene Editing, Oryza, Plants, Genetically Modified, CRISPR-Cas Systems
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