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Distinct functions of polysaccharide deacetylases in cell shape, neutral polysaccharide synthesis and virulence of Bacillus anthracis
Abstract
Summary: Peptidoglycan deacetylases (PGNG‐dacs) belong to the C arbohydrate E sterase F amily 4 (CE 4) and have been described as required for bacterial evasion to lysozyme and innate immune responses. Interestingly, there is an unusual occurrence of 10 putative polysaccharide deacetylases, including five PGNG‐dacs, in the B acillus sp. genomes, especially B. cereus and B. anthracis. To elucidate the physiological role of these multiple deacetylases, we employed genetic analysis and protein localization studies of five putative PGNG‐dacs from B. anthracis as well as biochemical analysis of their corresponding homologues from B. cereus. Our data confirm that three enzymes are PGNG‐dacs. While BA 1977, associated with lateral peptidoglycan synthesis, is a bona fide peptidoglycan deacetylase involved in resistance to host lysozyme and required for full virulence, BA 1961 and BA 3679 participate in the biogenesis of the peptidoglycan during both elongation and cell division. Furthermore, two enzymes are important for neutral polysaccharide attachment to PG and consequently anchoring of S‐layer proteins (BA 5436) and for polysaccharide modification (BA 2944). Our results provide novel and fundamental insights into the function of polysaccharide deacetylases in a major bioterrorism agent.
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Bacterial genes for polysaccharide deacetylase were enriched in responders.
“Using a new binning approach to focus on candidate bacterial driver genes, we observed enrichment of bacterial genes encoding key enzymes: polysaccharide deacetylase (associated with reduction of polysaccharide immunogenicity42) in Rs; and alpha-l-fucosidase45 and alpha-galactosidase46 (associated with mucus degradation) and glycosyltransferases (associated with LPS synthesis57) in NRs.”
From Intestinal microbiota signatures of clinical response and immune-related adverse events in melanoma patients treated with anti-PD-1 (2022) · cites this paper as [42]
Favorable taxa had more genes for polysaccharide deacetylation, iron transport, and iron-induced ROS production.
“Within favorable taxa, we identified increased abundance of genes related to polysaccharide deacetylation known to play a role in immune evasion42, as well as iron transport and iron-induced production of reactive oxygen species ROS, which affect mucosal healing43.”
From Intestinal microbiota signatures of clinical response and immune-related adverse events in melanoma patients treated with anti-PD-1 (2022) · cites this paper as [42]
Citations
Cites
A SIMPLE METHOD OF ESTIMATING FIFTY PER CENT ENDPOINTS
Reed, L., & Muench, H. (1938) · American Journal of Epidemiology · doi:10.1093/oxfordjournals.aje.a118408 · Cited by 17,629
A simple method of estimating 50 per cent end points
Muench, H. (1938) · Medical Entomology and Zoology · Cited by 8,376
Helgason, E., Økstad, O. A., Caugant, D. A., Johansen, H. A., Fouet, A., Mock, M., Hegna, I. K., & Kolstø, A. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.6.2627-2630.2000 · Cited by 1,072
Self-citationAnthrax as a Biological Weapon, 2002
Inglesby, T. V., O’Toole, T., Henderson, D. A., Bartlett, J. G., Ascher, M. S., Eitzen, E. M., Friedlander, A. M., Gerberding, J. L., HAUER, J. M., Hughes, J. M., et al. (2002) · JAMA · doi:10.1001/jama.287.17.2236 · Cited by 1,023
Mock, M., & Fouet, A. (2001) · Annual Review of Microbiology · doi:10.1146/annurev.micro.55.1.647 · Cited by 895
Self-citationControl of Cell Morphogenesis in Bacteria
Daniel, R. A., & Errington, J. (2003) · Cell · doi:10.1016/s0092-8674(03)00421-5 · Cited by 740
Vuong, C., Kocianova, S., Voyich, J. M., Yao, Y., Fischer, E. R., DeLeo, F. R., & Otto, M. J. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m411374200 · Cited by 592
Garner, E. C., Bernard, R., Wang, W., Zhuang, X., Rudner, D. Z., & Mitchison, T. J. (2011) · Science · doi:10.1126/science.1203285 · Cited by 526
Demonstration of a capsule plasmid in Bacillus anthracis
Green, B. D., Battisti, L., Koehler, T. M., Thorne, C. B., & Ivins, B. E. (1985) · Infection and Immunity · doi:10.1128/iai.49.2.291-297.1985 · Cited by 491
Boneca, I. G., Dussurget, O., Cabanes, D., Nahori, M., Sousa, S., Lecuit, M., Psylinakis, E., Bouriotis, V., Hugot, J., Giovannini, M., et al. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0609672104 · Cited by 378
Self-citationContribution of individual toxin components to virulence of Bacillus anthracis
Pezard, C., Berche, P., & Mock, M. (1991) · Infection and Immunity · doi:10.1128/iai.59.10.3472-3477.1991 · Cited by 371
Structural variation in the glycan strands of bacterial peptidoglycan
Vollmer, W. (2008) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2007.00088.x · Cited by 370
Mesnage, S., Fontaine, T., Mignot, T., Delepierre, M., Mock, M., & Fouet, A. (2000) · The EMBO Journal · doi:10.1093/emboj/19.17.4473 · Cited by 327
Self-citationPlasmid transfer by conjugation from Escherichia coli to Gram-positive bacteria
Trieu‐Cuot, P., Carlier, C., Martin, P., & Courvalin, P. M. (1987) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1987.tb02558.x · Cited by 290
The pgdA Gene Encodes for a PeptidoglycanN-Acetylglucosamine Deacetylase in Streptococcus pneumoniae
Vollmer, W., & Tomasz, A. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m910189199 · Cited by 269
The energized membrane and cellular autolysis in Bacillus subtilis
Jolliffe, L. K., Doyle, R. J., & Streips, U. N. (1981) · Cell · doi:10.1016/0092-8674(81)90183-5 · Cited by 256
Blair, D. E., Schüttelkopf, A. W., MacRae, J. I., & van Aalten, D. M. F. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0504339102 · Cited by 230
Trieu‐Cuot, P., Carlier, C., Poyart, C., & Courvalin, P. M. (1991) · Gene · doi:10.1016/0378-1119(91)90546-n · Cited by 210
The role of peptidoglycan in pathogenesis
Boneca, I. G. (2005) · Current Opinion in Microbiology · doi:10.1016/j.mib.2004.12.008 · Cited by 207
Self-citationModifications to the Peptidoglycan Backbone Help Bacteria To Establish Infection
Davis, K. M., & Weiser, J. N. (2010) · Infection and Immunity · doi:10.1128/iai.00651-10 · Cited by 196
Foster, S. J. (1992) · Journal of Bacteriology · doi:10.1128/jb.174.2.464-470.1992 · Cited by 172
Mesnage, S., Couture‐Tosi, E., Mock, M., Gounon, P., & Fouet, A. (1997) · Molecular Microbiology · doi:10.1046/j.1365-2958.1997.2941659.x · Cited by 153
Self-citationCharacterization of the Bacillus anthracis S-layer: cloning and sequencing of the structural gene
Etienne-Toumelin, I., Sirard, J., DUFLOT, E., Mock, M., & Fouet, A. (1995) · Journal of Bacteriology · doi:10.1128/jb.177.3.614-620.1995 · Cited by 150
Self-citationAnalysis of the peptidoglycan structure of Bacillus subtilis endospores
Popham, D. L., Helin, J., Costello, C. E., & Setlow, P. (1996) · Journal of Bacteriology · doi:10.1128/jb.178.22.6451-6458.1996 · Cited by 147
Carbohydrate esterase family 4 enzymes: substrate specificity
Caufrier, F., Martinou, A., Dupont, C., & Bouriotis, V. (2003) · Carbohydrate Research · doi:10.1016/s0008-6215(03)00002-8 · Cited by 138
Self-citationVollmer, W., & Tomasz, A. (2002) · Infection and Immunity · doi:10.1128/iai.70.12.7176-7178.2002 · Cited by 131
Murphy, E. (1985) · Molecular and General Genetics MGG · doi:10.1007/bf00383309 · Cited by 128
Significant contribution of the pgdA gene to the virulence of Streptococcus suis
Fittipaldi, N., Sekizaki, T., Takamatsu, D., Domínguez-Punaro, M. D. L. C., Harel, J., Bui, N. K., Vollmer, W., & Gottschalk, M. G. (2008) · Molecular Microbiology · doi:10.1111/j.1365-2958.2008.06463.x · Cited by 118
Chitlaru, T., Gat, O., Gozlan, Y., Ariel, N., & Avigdor Shafferman, C. (2006) · Journal of Bacteriology · doi:10.1128/jb.188.10.3551-3571.2006 · Cited by 117
Mesnage, S., Tosi‐Couture, E., & Fouet, A. (1999) · Molecular Microbiology · doi:10.1046/j.1365-2958.1999.01232.x · Cited by 105
Self-citationTrieu‐Cuot, P., Carlier, C., Poyart, C., & Courvalin, P. M. (1991) · Gene · doi:10.1016/0378-1119(91)90561-o · Cited by 104
Production of Muramic δ-Lactam in Bacillus subtilis Spore Peptidoglycan
Gilmore, M. E., Bandyopadhyay, D., Dean, A. M., et al. (2003) · Journal of Bacteriology · doi:10.1128/jb.186.1.80-89.2004 · Cited by 100
The Structure of the Major Cell Wall Polysaccharide of Bacillus anthracis Is Species-specific
Choudhury, B., Leoff, C., Saile, E., et al. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m605768200 · Cited by 89
Flouret, B., Mengin‐Lecreulx, D., & van HEIJENOORT, J. (1981) · Analytical Biochemistry · doi:10.1016/0003-2697(81)90451-6 · Cited by 86
Optimization of green fluorescent protein expression vectors for in vitro and in vivo detection of
Fortineau, N., Trieu‐Cuot, P., Gaillot, O., Pellegrini, E., Berche, P., & Gaillard, J. (2000) · Research in Microbiology · doi:10.1016/s0923-2508(00)00158-3 · Cited by 85
Gimenez, A. P., Wu, Y., Payá, M., et al. (2004) · The Journal of Immunology · doi:10.4049/jimmunol.173.1.521 · Cited by 84
Psylinakis, E., Boneca, I. G., Mavromatis, K., et al. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m407426200 · Cited by 83
Self-citationMouse Lysozyme M Is Important in Pulmonary Host Defense against Klebsiella pneumoniae Infection
Markart, P., Korfhagen, T. R., Weaver, T. E., & Akinbi, H. T. (2003) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.200305-669oc · Cited by 83
Benachour, A., Ladjouzi, R., Jeune, A. L., et al. (2012) · Journal of Bacteriology · doi:10.1128/jb.00981-12 · Cited by 82
Laaberki, M.-H., Pfeffer, J. M., Clarke, A. J., et al. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.183236 · Cited by 82
Kern, J. W., Ryan, C., Faull, K. F., & Schneewind, O. (2010) · Journal of Molecular Biology · doi:10.1016/j.jmb.2010.06.059 · Cited by 80
Peptidoglycan N-acetylglucosamine deacetylation decreases autolysis in Lactococcus lactis
Meyrand, M., Boughammoura, A., Courtin, P., Mézange, C., Guillot, A., & Chapot, M.-P. (2007) · Microbiology · doi:10.1099/mic.0.2007/005835-0 · Cited by 78
The surface of Bacillus anthracis
Fouet, A. (2009) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2009.07.001 · Cited by 75
Self-citationSchirner, K., & Errington, J. (2008) · Journal of Bacteriology · doi:10.1128/jb.01497-08 · Cited by 67
Potluri, L., de Pedro, M. A., & Young, K. D. (2012) · Molecular Microbiology · doi:10.1111/j.1365-2958.2012.08023.x · Cited by 64
Milani, C. J., Aziz, R. K., Locke, J. B., Dahesh, S., Nizet, V., & Buchanan, J. T. (2009) · Microbiology · doi:10.1099/mic.0.028365-0 · Cited by 61
Detailed Structural Analysis of the Peptidoglycan of the Human Pathogen Neisseria meningitidis
Antignac, A., Rousselle, J.-C., Namane, A., Labigne-Roussel, A., Taha, M., & Boneca, I. G. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m304749200 · Cited by 60
Self-citationThe extracellular and cytoplasmic proteomes of the non‐virulent Bacillus anthracis strain UM23C1‐2
Antelmann, H., Williams, R. C., Miethke, M., Wipat, A., Albrecht, D., Harwood, C., & Hecker, M. (2005) · Proteomics · doi:10.1002/pmic.200401218 · Cited by 59
Fukushima, T., Kitajima, T., & Sekiguchi, J. (2005) · Journal of Bacteriology · doi:10.1128/jb.187.4.1287-1292.2005 · Cited by 53
Kobayashi, K., Sudiarta, I. P., Kodama, T., Fukushima, T., Ara, K., Ozaki, K., & Sekiguchi, J. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.329490 · Cited by 52
Forsberg, L. S., Choudhury, B., Leoff, C., et al. (2011) · Glycobiology · doi:10.1093/glycob/cwr026 · Cited by 45
Padua, L. (1990) · FEMS Microbiology Letters · doi:10.1016/0378-1097(90)90293-y · Cited by 40
Isolation and purification of cell wall polysaccharide ofBacillus anthracis(Δ Sterne)
Ekwunife, F. S., Singh, J., Taylor, K. G., & Doyle, R. J. (1991) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1991.tb04891.x · Cited by 37
Leoff, C., Choudhury, B., Saile, E., et al. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m803234200 · Cited by 35
Deng, D., Urch, J., ten Cate, J. M., Rao, V. A., van Aalten, D. M. F., & Wim Crielaard, A. (2008) · Journal of Bacteriology · doi:10.1128/jb.00838-08 · Cited by 33
Plasmid-Encoded Autolysin inBacillus anthracis: Modular Structure and Catalytic Properties
Mesnage, S., & Fouet, A. (2002) · Journal of Bacteriology · doi:10.1128/jb.184.1.331-334.2002 · Cited by 28
Self-citationEnzymatic Deacetylation of N-Acetylglucosamine Residues in Cell Wall Peptidoglycan
Araki, Y., oba, S., Ito, E., et al. (1980) · The Journal of Biochemistry · doi:10.1093/oxfordjournals.jbchem.a132994 · Cited by 27
Kaoukab-Raji, A., Biskri, L., Bernardini, M. L., & Allaoui, A. (2012) · Microbes and Infection · doi:10.1016/j.micinf.2012.01.009 · Cited by 25
Leoff, C., Saile, E., Rauvolfová, J., et al. (2009) · Glycobiology · doi:10.1093/glycob/cwp036 · Cited by 25
Co-existence ofclpBandclpCin the Bacillaceae
Namy, O., Mock, M. ̈., & Fouet, A. (1999) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1999.tb13517.x · Cited by 14
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Research works printed but not linked: 2
- Reed L.J., 1938, A simple method of estimating fifty percent endpoints, Am J Hyg, 27, 493
- US Department of Health and Human Services National Institute of Allergy and Infectious Diseases (NIAID)(2006)Biodefense Research Agenda for CDC Category A Agents:Progress Report pp.15–23.
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McCulloch, J. A., Davar, D., Rodrigues, R. R., Badger, J. H., Fang, J. R., Cole, A. M., Balaji, A. K., Vetizou, M., Prescott, S. M., Fernandes, M. R., et al. (2022) · Nature Medicine · doi:10.1038/s41591-022-01698-2 · Cited by 472
Metadata
- Authors
- Balomenou, S., Fouet, A., Tzanodaskalaki, M., Couture‐Tosi, E., Bouriotis, V., & Boneca, I. G.
- Affiliations
- University of Crete (GR)
- Institut Pasteur Unite Toxines et Pathogénie Bacteriénnes 25‐28 rue de Dr Roux 75724 Paris Cedex 15 France
- FORTH Institute of Molecular Biology and Biotechnology (GR)
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Corresponding author
- Bouriotis, Vassilis
- Boneca, Ivo G.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2013-01-21
- Volume
- 87
- Issue
- 4
- Pages
- 867-883
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W1951059500
- Cited by
- 49
- References
- 60
- Citations by year
- 2025: 2 · 2024: 4 · 2023: 3 · 2022: 1 · 2021: 4 · 2020: 6 · 2019: 4 · 2018: 8 · 2017: 4 · 2015: 5 · 2014: 7 · 2013: 1
- FWCI
- 1.94
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacillus and Francisella bacterial research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Keywords
- polysaccharide deacetylase, Bacillus anthracis, Bacillus cereus, lysozyme resistance, bacterial virulence, S-layer proteins, cell shape, cell division, bioterrorism agents
- MeSH terms
- Amidohydrolases, Animals, Anthrax, Bacillus anthracis, Bacterial Proteins, Female, Humans, Male, Mice, Inbred BALB C, Polysaccharides, Bacterial, Virulence, Protein Transport, Mice
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