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Cited by 143Bacterial Genetics and BiotechnologyBacterial biofilms and quorum sensingBiochemical and Structural Characterization

The DOI matches its Crossref record; 98 of the 98 references it cites were found in a registry or an index.

A mechanical signal transmitted by the flagellum controls signalling in Bacillus subtilis

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Cairns, Lynne S., Marlow, Victoria L., Bissett, Emma, Ostrowski, Adam, Stanley‐Wall, Nicola R.·2013·Molecular Microbiology
DOI 10.1111/mmi.12342PMC PMC3963450
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Abstract

Summary: In the natural environment bacteria predominantly live adhered to a surface as part of a biofilm. While many of the components needed for biofilm assembly are known, the mechanism by which microbes sense and respond to contact with a surface is poorly understood. B acillus subtilis is a G ram‐positive model for biofilm formation. The DegS – DegU two‐component system controls several multicellular behaviours in B . subtilis , including biofilm formation. Here we identify the B . subtilis flagellum as a mechanosensor that activates the DegS – DegU regulatory pathway. Inhibition of flagellar rotation by deletion or mutation of the flagellar stator gene, motB , results in an increase in both degU transcription and DegU ∼ P driven processes, namely exoprotease production and poly‐γ‐ dl ‐glutamic acid biosynthesis. Similarly, inhibition of flagellar rotation by engaging the flagellar clutch or by tethering the flagella with antibodies also promotes an increase in degU transcription that is reflective of increased DegU ∼ P levels in the cell. Collectively, these findings strongly indicate that inhibition of flagellar rotation acts as a mechanical trigger to activate the DegS – DegU two‐component signal transduction system. We postulate that inhibition of flagellar rotation could function as a mechanical trigger to activate bacterial signal transduction cascades in many motile bacteria upon contact with a surface.

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  1. 10.1073/pnas.130639110 · DOI

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Metadata

Authors
Cairns, L. S., Marlow, V. L., Bissett, E., Ostrowski, A., & Stanley‐Wall, N. R.
Affiliations
University of Dundee (GB)
Corresponding author
Stanley‐Wall, Nicola R.
ORCID
Venue
Molecular Microbiology
Publisher
Wiley
Published
2013-08-14
Volume
90
Issue
1
Pages
6-21
ISSN
  • 0950-382X (ISSN-L)
  • 1365-2958
DOI
10.1111/mmi.12342
PMC
PMC3963450
OpenAlex
W1908456294
Cited by
143
References
98
Citations by year
2026: 7 · 2025: 8 · 2024: 7 · 2023: 7 · 2022: 9 · 2021: 13 · 2020: 17 · 2019: 12 · 2018: 6 · 2017: 12 · 2016: 13 · 2015: 13 · 2014: 11 · 2013: 8
FWCI
9.95
Type
journal article
Language
en
Open access
Licence
Locations
  • Molecular Microbiology (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Discovery Research Portal (University of Dundee) (submittedVersion)
Funders
  • Wellcome Trust (093714, 097945, 093714/Z/10/Z, 097818/Z/11, 097945/B/11/Z, 097818/Z/11/A)
  • International Seafood Sustainability Foundation
  • Directorate for Biological Sciences
  • Biotechnology and Biological Sciences Research Council (BB/I006915/1, BB/H002340/1, BB/D526161/1)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
Keywords
Bacillus subtilis, flagella, mechanosensing, biofilm formation, MotB, bacterial signal transduction
MeSH terms
Bacillus subtilis, Bacterial Proteins, Flagella, Locomotion, Signal Transduction, Gene Expression Regulation, Bacterial

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