Deoxycholic acid induced changes in electrophysiological parameters and macromolecular permeability in murine small intestine with and without functional enteric nervous system plexuses
Abstract
Background: We have previously shown in mice that the fecal proportion and concentration of the hydrophobic bile acid deoxycholic acid ( DCA ) is elevated with high‐fat feeding and that these changes are able to disrupt the intestinal barrier function. The aim of this study was to investigate whether these changes are mediated by the enteric nervous system ( ENS ). Methods: The function of the ENS in the small intestinal tissues of mice was compromised by two different methods: by removing the seromuscular layer and by incubating the intact tissues with tetrodotoxin ( TTX ), a neural conduction blocker, before DCA treatment. Tissues with or without functional plexuses were mounted into a U ssing chamber system and treated with 3 mM DCA for 20 min. After DCA treatment, the intestinal permeability to fluorescein was assessed. Short‐circuit current (I sc ) and transepithelial resistance ( TER ) were recorded throughout the experiment. Key Results: DCA increased intestinal fluorescein permeability only in tissues where the seromuscular layer was removed. In tissues with intact seromuscular layer, DCA induced a significant increase in TER , which was attenuated by blocking of the neural function by TTX . Conclusions & Inferences: The results of this study suggest that the DCA ‐induced increase observed in fluorescein permeability is not mediated through neural pathways, but more due to a direct effect on the epithelium. However, as TTX was able to attenuate the DCA ‐induced increase in TER , it can be speculated that DCA is also able to elicit responses through neural pathways.
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Microbial metabolites such as bile acids and SCFAs can regulate intestinal permeability.
“Microbial metabolites such as bile acids83,84 and SCFAs can also regulate intestinal permeability (Table 1).”
From The gut Microbiome in adult and pediatric functional gastrointestinal disorders (2019) · cites this paper as [84]
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The gut Microbiome in adult and pediatric functional gastrointestinal disorders
Shin, A., Preidis, G. A., Shulman, R., & Kashyap, P. C. (2019) · Clinical Gastroenterology and Hepatology · doi:10.1016/j.cgh.2018.08.054 · Cited by 163 · see citing works
- Authors
- Forsgård, R. A., Korpela, R., Stenman, L. K., Österlund, P., & Holma, R.
- Affiliations
- University of Helsinki (FI)
- Department of Oncology Helsinki University Central Hospital HUS Helsinki Finland
- Corresponding author
- Holma, R.
- Venue
- Neurogastroenterology & Motility
- Publisher
- Wiley
- Published
- 2014-06-23
- Volume
- 26
- Issue
- 8
- Pages
- 1179-1187
- ISSN
- 1350-1925 (ISSN-L)
- 1365-2982
- OpenAlex
- W2042819483
- Cited by
- 20
- References
- 36
- Citations by year
- 2026: 2 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 5 · 2019: 2 · 2018: 4 · 2016: 1
- FWCI
- 0.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neurogastroenterology & Motility (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Tekes (40482/09)
- Foundation of Clinical Chemistry Research
- Topics
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Keywords
- deoxycholic acid, bile acids, enteric nervous system, small intestine, intestinal permeability, intestinal barrier function, tetrodotoxin, transepithelial electrical resistance, short-circuit current, fluorescein permeability, neural pathways, high-fat feeding
- MeSH terms
- Animals, Deoxycholic Acid, Intestinal Mucosa, Intestine, Small, Male, Mice, Inbred C57BL, Permeability, Enteric Nervous System, Fluorescein, Mice, Electrophysiological Phenomena
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