The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Increasing water‐use efficiency directly through genetic manipulation of stomatal density
Abstract
Summary: Improvement in crop water‐use efficiency (WUE) is a critical priority for regions facing increased drought or diminished groundwater resources. Despite new tools for the manipulation of stomatal development, the engineering of plants with high WUE remains a challenge. We used Arabidopsis epidermal patterning factor (EPF) mutants exhibiting altered stomatal density to test whether WUE could be improved directly by manipulation of the genes controlling stomatal density. Specifically, we tested whether constitutive overexpression of EPF 2 reduced stomatal density and maximum stomatal conductance (gw(max)) sufficiently to increase WUE. We found that a reduction in gw(max) via reduced stomatal density in EPF 2‐overexpressing plants (EPF 2OE) increased both instantaneous and long‐term WUE without altering significantly the photosynthetic capacity. Conversely, plants lacking both EPF 1 and EPF 2 expression (epf1epf2) exhibited higher stomatal density, higher gw(max) and lower instantaneous WUE, as well as lower (but not significantly so) long‐term WUE. Targeted genetic modification of stomatal conductance, such as in EPF 2OE, is a viable approach for the engineering of higher WUE in crops, particularly in future high‐carbon‐dioxide (CO2) atmospheres.
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Cites
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Self-citation
Cited by
Impact of stomatal density and morphology on water-use efficiency in a changing world
Bertolino, L. T., Caine, R. S., & Gray, J. E. (2019) · Frontiers in Plant Science · doi:10.3389/fpls.2019.00225 · Cited by 706
Self-citationCaine, R. S., Yin, X., Sloan, J., Harrison, E. L., Mohammed, U., Fulton, T., Biswal, A. K., Dionora, J., Chater, C. C., Coe, R. A., et al. (2019) · New Phytologist · doi:10.1111/nph.15344 · Cited by 561
Self-citationVvEPFL9-1 knock-out via CRISPR/Cas9 reduces stomatal density in grapevine
Clemens, M., Faralli, M., Lagreze, J., Bontempo, L., Piazza, S., Varotto, C., Malnoy, M., Oechel, W., Rizzoli, A., & Dalla Costa, L. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.878001 · Cited by 57
Crops, Nitrogen, Water: Are Legumes Friend, Foe, or Misunderstood Ally?
Adams, M. A., Buchmann, N., Sprent, J., Buckley, T. N., & Turnbull, T. L. (2018) · Trends in Plant Science · doi:10.1016/j.tplants.2018.02.009 · Cited by 54
Metadata
- Authors
- Franks, P. J., W. Doheny‐Adams, T., Britton‐Harper, Z. J., & Gray, J. E.
- Venue
- New Phytologist
- Publisher
- Wiley
- Published
- 2015-03-09
- Volume
- 207
- Issue
- 1
- Pages
- 188-195
- ISSN
- 0028-646X (ISSN-L)
- 1469-8137
- OpenAlex
- W2027778839
- Cited by
- 391
- References
- 38
- FWCI
- 20.42
- Type
- journal article
- Language
- en
- Open access
- closed
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- Franks, P. J., W. Doheny‐Adams, T., Britton‐Harper, Z. J., & Gray, J. E. (2015). Increasing water‐use efficiency directly through genetic manipulation of stomatal density. New Phytologist. https://doi.org/10.1111/nph.13347
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