The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
The cbfs triple mutants reveal the essential functions of CBF s in cold acclimation and allow the definition of CBF regulons in Arabidopsis
Open accessAbstract
Summary: In Arabidopsis, the C‐repeat binding factors (CBF s) have been extensively studied as key transcription factors in the cold stress response. However, their exact functions in the cold response remains unclear due to the lack of a null cbf triple mutant. In this study, we used CRISPR/Cas9 technology to mutate CBF 1 or CBF 1/CBF 2 in a cbf3 T‐DNA insertion mutant to generate cbf1,3 double and cbf1 cbf2 cbf3 (cbfs) triple mutants. The response of the cbfs triple mutants to chilling stress is impaired. Furthermore, no significant difference in freezing tolerance was observed between the wild‐type and the cbf1,3 and cbfs mutants without cold acclimation. However, the cbfs mutants were extremely sensitive to freezing stress after cold acclimation, and freezing sensitivity ranking was cbfs > cbf1,3 > cbf3. RNA‐Seq analysis showed that 134 genes were CBF regulated, of which 112 were regulated positively and 22 negatively by CBF s. Our study reveals the essential functions of CBF s in chilling stress response and cold acclimation, as well as defines a set of genes as CBF regulon. It also provides materials for the genetic dissection of components in CBF‐dependent cold signaling.
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Ding, Y., Shi, Y., & Yang, S. (2020) · Molecular Plant · doi:10.1016/j.molp.2020.02.004 · Cited by 787
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Self-citationSurviving and thriving: How plants perceive and respond to temperature stress
Ding, Y., & Yang, S. (2022) · Developmental Cell · doi:10.1016/j.devcel.2022.03.010 · Cited by 396
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Wang, X., Tu, M., Wang, D., Liu, J., Li, Y., Li, Z., Wang, Y., & Wang, X. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.12832 · Cited by 367
Metadata
- Authors
- Jia, Y., Ding, Y., Shi, Y., Zhang, X., Gong, Z., & Yang, S.
- Venue
- New Phytologist
- Publisher
- Wiley
- Published
- 2016-06-29
- Volume
- 212
- Issue
- 2
- Pages
- 345-353
- ISSN
- 0028-646X (ISSN-L)
- 1469-8137
- OpenAlex
- W2472257420
- Cited by
- 481
- References
- 6
- FWCI
- 29.93
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Jia, Y., Ding, Y., Shi, Y., Zhang, X., Gong, Z., & Yang, S. (2016). The cbfs triple mutants reveal the essential functions of CBF s in cold acclimation and allow the definition of CBF regulons in Arabidopsis. New Phytologist. https://doi.org/10.1111/nph.14088
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