HTSeq—a Python framework to work with high-throughput sequencing data
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Abstract
Motivation: A large choice of tools exists for many standard tasks in the analysis of high-throughput sequencing (HTS) data. However, once a project deviates from standard workflows, custom scripts are needed. Results: We present HTSeq, a Python library to facilitate the rapid development of such scripts. HTSeq offers parsers for many common data formats in HTS projects, as well as classes to represent data, such as genomic coordinates, sequences, sequencing reads, alignments, gene model information and variant calls, and provides data structures that allow for querying via genomic coordinates. We also present htseq-count, a tool developed with HTSeq that preprocesses RNA-Seq data for differential expression analysis by counting the overlap of reads with genes. Availability and implementation: HTSeq is released as an open-source software under the GNU General Public Licence and available from http://www-huber.embl.de/HTSeq or from the Python Package Index at https://pypi.python.org/pypi/HTSeq . Contact: sanders@fs.tum.de
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Algorithms that quantify expression from transcriptome mappings allocate multi-mapping reads among transcripts and output within-sample normalized values corrected for sequencing biases.
“These methods allocate multi-mapping reads among transcript and output within-sample normalized values corrected for sequencing biases [35, 41, 43].”
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From limma powers differential expression analyses for RNA-sequencing and microarray studies (2015) · cites this paper as [30]
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- Authors
- Anders, S., Pyl, P. T., & Huber, W.
- Affiliations
- European Molecular Biology Laboratory (DE)
- Corresponding author
- Anders, Simon
- Venue
- Bioinformatics
- Publisher
- Oxford University Press (OUP)
- Published
- 2014-09-25
- Volume
- 31
- Issue
- 2
- Pages
- 166-169
- ISSN
- 1367-4803 (ISSN-L)
- 1367-4811
- PMC
- PMC4287950
- OpenAlex
- W2134526812
- Cited by
- 21,464
- References
- 16
- Citations by year
- 2026: 1267 · 2025: 1823 · 2024: 2188 · 2023: 2215 · 2022: 2453 · 2021: 2749 · 2020: 2323 · 2019: 1943 · 2018: 1658 · 2017: 1272 · 2016: 901 · 2015: 516 · 2014: 134 · 2013: 13
- FWCI
- 383.82
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Bioinformatics (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- European Commission (305626)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
- Keywords
- HTSeq, high-throughput sequencing data, Python library, RNA-seq, read counting, differential expression analysis, variant calls, gene models
- MeSH terms
- Gene Expression Regulation, Humans, Software, Genome, Human, Genomics, High-Throughput Nucleotide Sequencing
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