Interactions between Streptococcus oralis, Actinomyces oris, and Candida albicans in the development of multispecies oral microbial biofilms on salivary pellicle
Open accessAbstract
Summary: The fungus Candida albicans is carried orally and causes a range of superficial infections that may become systemic. Oral bacteria Actinomyces oris and Streptococcus oralis are abundant in early dental plaque and on oral mucosa. The aims of this study were to determine the mechanisms by which S. oralis and A. oris interact with each other and with C. albicans in biofilm development. Spatial distribution of microorganisms was visualized by confocal laser scanning microscopy of biofilms labeled by differential fluorescence or by fluorescence in situ hybridization (FISH). Actinomyces oris and S. oralis formed robust dual‐species biofilms, or three‐species biofilms with C. albicans. The bacterial components tended to dominate the lower levels of the biofilms while C. albicans occupied the upper levels. Non‐fimbriated A. oris was compromised in biofilm formation in the absence or presence of streptococci, but was incorporated into upper biofilm layers through binding to C. albicans. Biofilm growth and hyphal filament production by C. albicans was enhanced by S. oralis. It is suggested that the interkingdom biofilms are metabolically coordinated to house all three components, and this study demonstrates that adhesive interactions between them determine spatial distribution and biofilm architecture. The physical and chemical communication processes occurring in these communities potentially augment C. albicans persistence at multiple oral cavity sites.
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The oral microbiome: Role of key organisms and complex networks in oral health and disease
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- Authors
- Cavalcanti, I. M. G., Del Bel Cury, A. A., Jenkinson, H. F., & Nobbs, A. H.
- Affiliations
- Universidade Estadual de Campinas (UNICAMP) (BR)
- Department of Prosthodontics and Periodontology Piracicaba Dental School – University of Campinas Piracicaba São Paulo Brazil
- University of Bristol (GB)
- Corresponding author
- Nobbs, A.H.
- Venue
- Molecular Oral Microbiology
- Publisher
- Wiley
- Published
- 2016-03-15
- Volume
- 32
- Issue
- 1
- Pages
- 60-73
- ISSN
- 2041-1006 (ISSN-L)
- 2041-1014
- OpenAlex
- W2255985866
- Cited by
- 62
- References
- 61
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 10 · 2023: 3 · 2022: 7 · 2021: 6 · 2020: 6 · 2019: 9 · 2018: 7 · 2017: 2 · 2016: 3
- FWCI
- 10.43
- Type
- journal article
- Language
- en
- Open access
- Licence
- Locations
- Molecular Oral Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Explore Bristol Research (acceptedVersion, other-oa)
- Bristol Research (University of Bristol) (acceptedVersion, other-oa)
- Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo (2013/15884‐1)
- Topics
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Keywords
- Streptococcus oralis, Actinomyces oris, Candida albicans, polymicrobial biofilms, salivary pellicle, cross-kingdom interactions, dual-species biofilms, biofilm structure, hyphal development, bacterial adhesion, fimbriae, dental plaque, oral microbiota
- MeSH terms
- Actinomyces, Bacterial Adhesion, Candida albicans, Dental Plaque, Humans, Mouth, Mouth Mucosa, In Situ Hybridization, Fluorescence, Biofilms, Microscopy, Confocal, Streptococcus oralis, Dental Pellicle, Microbial Interactions
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