Optimization of TaDREB3 gene expression in transgenic barley using cold‐inducible promoters
Open accessAbstract
Summary: Constitutive over‐expression of the TaDREB3 gene in barley improved frost tolerance of transgenic plants at the vegetative stage of plant development, but leads to stunted phenotypes and 3‐ to 6‐week delays in flowering compared to control plants. In this work, two cold‐inducible promoters with contrasting properties, the WRKY 71 gene promoter from rice and the C or39 gene promoter from durum wheat, were applied to optimize expression of TaDREB3 . The aim of the work was to increase plant frost tolerance and to decrease or prevent negative developmental phenotypes observed during constitutive expression of TaDREB3 . The OsWRKY71 and TdCor39 promoters had low‐to‐moderate basal activity and were activated by cold treatment in leaves, stems and developing spikes of transgenic barley and rice. Expression of the TaDREB3 gene, driven by either of the tested promoters, led to a significant improvement in frost tolerance. The presence of the functional TaDREB3 protein in transgenic plants was confirmed by the detection of strong up‐regulation of cold‐responsive target genes. The OsWRKY71 promoter–driven TaDREB3 provides stronger activation of the same target genes than the TdCor39 promoter. Analysis of the development of transgenic plants in the absence of stress revealed small or no differences in plant characteristics and grain yield compared with wild‐type plants. The WRKY71– TaDREB3 promoter–transgene combination appears to be a promising tool for the enhancement of cold and frost tolerance in crop plants but field evaluation will be needed to confirm that negative development phenotypes have been controlled.
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In transgenic barley, use of stress-inducible or modified promoters has been shown to address abnormal growth associated with constitutive overexpression.
“In transgenic barley, the use of stress-inducible or modified promoters has been shown to address abnormal growth associated with constitutive overexpression and could plausibly be applied here (Kovalchuk et al., 2013).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Kovalchuk et al., 2013
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Karunarathne, S., Walker, E., Sharma, D., Li, C., & Han, Y. (2023) · Journal of Zhejiang University-SCIENCE B · doi:10.1631/jzus.b2200552 · Cited by 18 · see citing works
“In transgenic barley, the use of stress-inducible or modified promot‐ ers has been shown to address abnormal growth asso‐ ciated with constitutive overexpression and could plau‐ sibly be applied here (Kovalchuk et al., 2013).”
- Authors
- Kovalchuk, N., Jia, W., Eini, O., Morran, S., Pyvovarenko, T., Fletcher, S., Bazanova, N., Harris, J., Beck‐Oldach, K., Shavrukov, Y., Langridge, P., & Lopato, S.
- Affiliations
- Australian Centre for Plant Functional Genomics (AU)
- Corresponding author
- Lopato, Sergiy
- Venue
- Plant Biotechnology Journal
- Publisher
- Wiley
- Published
- 2013-03-16
- Volume
- 11
- Issue
- 6
- Pages
- 659-670
- ISSN
- 1467-7644 (ISSN-L)
- 1467-7652
- OpenAlex
- W1970036241
- Cited by
- 81
- References
- 63
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 4 · 2023: 6 · 2022: 6 · 2021: 6 · 2020: 9 · 2019: 6 · 2018: 7 · 2017: 9 · 2016: 7 · 2015: 10 · 2014: 2 · 2013: 3
- FWCI
- 8.84
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Plant Biotechnology Journal (publishedVersion)
- PubMed (publishedVersion)
- Adelaide Research & Scholarship (AR&S) (University of Adelaide) (submittedVersion)
- Funders
- Government of South Australia
- Grains Research and Development Corporation
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- barley, frost resistance, transgenic plants, constitutive overexpression, cold stress, flowering time, stunting, grain yield, rice, durum wheat
- MeSH terms
- Adaptation, Physiological, Hordeum, Cloning, Molecular, Cold Temperature, Crosses, Genetic, Homozygote, Phenotype, Plant Proteins, Promoter Regions, Genetic, Triticum, Genes, Plant, Gene Expression Regulation, Plant, Gene Dosage, Transgenes, Plants, Genetically Modified, Flowers
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