The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Improving cold storage and processing traits in potato through targeted gene knockout
Open accessAbstract
Summary: Cold storage of potato tubers is commonly used to reduce sprouting and extend postharvest shelf life. However, cold temperature stimulates the accumulation of reducing sugars in potato tubers. Upon high‐temperature processing, these reducing sugars react with free amino acids, resulting in brown, bitter‐tasting products and elevated levels of acrylamide—a potential carcinogen. To minimize the accumulation of reducing sugars, RNA interference (RNAi) technology was used to silence the vacuolar invertase gene ( VInv ), which encodes a protein that breaks down sucrose to glucose and fructose. Because RNAi often results in incomplete gene silencing and requires the plant to be transgenic, here we used transcription activator‐like effector nucleases (TALENs) to knockout VInv within the commercial potato variety, Ranger Russet. We isolated 18 plants containing mutations in at least one VInv allele, and five of these plants had mutations in all VInv alleles. Tubers from full VInv‐ knockout plants had undetectable levels of reducing sugars, and processed chips contained reduced levels of acrylamide and were lightly coloured. Furthermore, seven of the 18 modified plant lines appeared to contain no TALEN DNA insertions in the potato genome. These results provide a framework for using TALENs to quickly improve traits in commercially relevant autotetraploid potato lines.
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Cites
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“Previous studies have indicated that off-target mutagenesis can occur when using TALENs for genome modification (Frock et al., 2015; Mali et al., 2013).”
BackgroundAcrylamide from Maillard Reaction Products
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Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases
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Improved soybean oil quality by targeted mutagenesis of the fatty acid desaturase 2 gene family
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Cited by
Wang, F., Wang, C., Liu, P., Lei, C., Hao, W., Gao, Y., Liu, Y.-G., & Zhao, K. (2016) · PLOS ONE · doi:10.1371/journal.pone.0154027 · Cited by 819
Genetically modified crops: current status and future prospects
Kumar, K., Gambhir, G., Dass, A., Tripathi, A. K., Singh, A., Jha, A. K., Yadava, P., Choudhary, M., & Rakshit, S. (2020) · Planta · doi:10.1007/s00425-020-03372-8 · Cited by 528
Genome editing in potato via CRISPR‐Cas9 ribonucleoprotein delivery
Andersson, M., Turesson, H., Olsson, N., Fält, A., Ohlsson, P., Gonzalez, M. N., Samuelsson, M., & Hofvander, P. (2018) · Physiologia Plantarum · doi:10.1111/ppl.12731 · Cited by 432
De Novo Domestication: An Alternative Route toward New Crops for the Future
Fernie, A. R., & Yan, J. (2019) · Molecular Plant · doi:10.1016/j.molp.2019.03.016 · Cited by 398
Consumer acceptance of food crops developed by genome editing
Ishii, T., & Araki, M. (2016) · Plant Cell Reports · doi:10.1007/s00299-016-1974-2 · Cited by 186
Metadata
- Authors
- Clasen, B. M., Stoddard, T. J., Luo, S., Demorest, Z. L., Li, J., Cedrone, F., Tibebu, R., Davison, S., Ray, E. E., Daulhac, A., Coffman, A., Yabandith, A., Retterath, A., Haun, W., Baltes, N. J., Mathis, L., Voytas, D. F., & Zhang, F.
- Venue
- Plant Biotechnology Journal
- Publisher
- Wiley
- Published
- 2015-04-07
- Volume
- 14
- Issue
- 1
- Pages
- 169-176
- ISSN
- 1467-7644 (ISSN-L)
- 1467-7652
- OpenAlex
- W2130103528
- Cited by
- 414
- References
- 30
- FWCI
- 45.58
- Type
- journal article
- Language
- en
- Open access
- green
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- Clasen, B. M., Stoddard, T. J., Luo, S., Demorest, Z. L., Li, J., Cedrone, F., Tibebu, R., Davison, S., Ray, E. E., Daulhac, A., Coffman, A., Yabandith, A., Retterath, A., Haun, W., Baltes, N. J., Mathis, L., Voytas, D. F., & Zhang, F. (2016). Improving cold storage and processing traits in potato through targeted gene knockout. Plant Biotechnology Journal. https://doi.org/10.1111/pbi.12370
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