The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Improved soybean oil quality by targeted mutagenesis of the fatty acid desaturase 2 gene family
Open accessAbstract
Summary: Soybean oil is high in polyunsaturated fats and is often partially hydrogenated to increase its shelf life and improve oxidative stability. The trans‐fatty acids produced through hydrogenation pose a health threat. Soybean lines that are low in polyunsaturated fats were generated by introducing mutations in two fatty acid desaturase 2 genes ( FAD 2‐1A and FAD 2‐1B ), which in the seed convert the monounsaturated fat, oleic acid, to the polyunsaturated fat, linoleic acid. Transcription activator‐like effector nucleases ( TALEN s) were engineered to recognize and cleave conserved DNA sequences in both genes. In four of 19 transgenic soybean lines expressing the TALEN s, mutations in FAD 2‐1A and FAD 2‐1B were observed in DNA extracted from leaf tissue; three of the four lines transmitted heritable FAD 2 ‐1 mutations to the next generation. The fatty acid profile of the seed was dramatically changed in plants homozygous for mutations in both FAD 2‐1A and FAD 2‐1B : oleic acid increased from 20% to 80% and linoleic acid decreased from 50% to under 4%. Further, mutant plants were identified that lacked the TALEN transgene and only carried the targeted mutations. The ability to create a valuable trait in a single generation through targeted modification of a gene family demonstrates the power of TALEN s for genome engineering and crop improvement.
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Cites
Zuo, J., Niu, Q., & Chua, N. (2000) · The Plant Journal · doi:10.1046/j.1365-313x.2000.00868.x · Cited by 1,177
Fatty Acids in Foods and their Health Implications
(2007) · CRC Press eBooks · doi:10.1201/9781420006902 · Cited by 1,079
Soybean Oil: Genetic Approaches for Modification of Functionality and Total Content
Clemente, T. E., & Cahoon, E. B. (2009) · Plant Physiology · doi:10.1104/pp.109.146282 · Cited by 613
Trans Fatty Acids and Coronary Heart Disease
Ascherio, A., Katan, M. B., Zock, P. L., Stampfer, M. J., & Willett, W. C. (1999) · New England Journal of Medicine · doi:10.1056/nejm199906243402511 · Cited by 569
Oleic acid content is responsible for the reduction in blood pressure induced by olive oil
Terés, S., Barceló-Coblijn, G., Benet, M., Álvarez, R., Bressani, R., Halver, J. E., & Escribá, P. V. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0807500105 · Cited by 518
Plant Genome Engineering with Sequence-Specific Nucleases
Voytas, D. F. (2013) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-042811-105552 · Cited by 446
Self-citationTargeted Mutagenesis of Duplicated Genes in Soybean with Zinc-Finger Nucleases
Curtin, S. J., Zhang, F., Sander, J. D., Haun, W. J., Starker, C., Baltes, N. J., Reyon, D., Dahlborg, E. J., Goodwin, M. J., Coffman, A. P., et al. (2011) · Plant Physiology · doi:10.1104/pp.111.172981 · Cited by 332
Self-citationPhenotypic and Genomic Analyses of a Fast Neutron Mutant Population Resource in Soybean
Bolon, Y.-T., Haun, W. J., Xu, W. W., Grant, D., Stacey, M. G., Nelson, R. T., Gerhardt, D. J., Jeddeloh, J. A., Stacey, G., Muehlbauer, G. J., et al. (2011) · Plant Physiology · doi:10.1104/pp.110.170811 · Cited by 201
Self-citationTang, G., Novitzky, W. P., Carol Griffin, H., Huber, S. C., & Dewey, R. E. (2005) · The Plant Journal · doi:10.1111/j.1365-313x.2005.02535.x · Cited by 201
Arnould, S., Chames, P., Perez, C., Lacroix, E., Duclert, A., Epinat, J.-C., Stricher, F., Petit, A.-S., Patin, A., Guillier, S., et al. (2006) · Journal of Molecular Biology · doi:10.1016/j.jmb.2005.10.065 · Cited by 191
Cited by
Wang, F., Wang, C., Liu, P., Lei, C., Hao, W., Gao, Y., Liu, Y.-G., & Zhao, K. (2016) · PLOS ONE · doi:10.1371/journal.pone.0154027 · Cited by 819
Targeted genome modifications in soybean with CRISPR/Cas9
Jacobs, T. B., LaFayette, P. R., Schmitz, R. J., & Parrott, W. A. (2015) · BMC Biotechnology · doi:10.1186/s12896-015-0131-2 · Cited by 577
Genetically modified crops: current status and future prospects
Kumar, K., Gambhir, G., Dass, A., Tripathi, A. K., Singh, A., Jha, A. K., Yadava, P., Choudhary, M., & Rakshit, S. (2020) · Planta · doi:10.1007/s00425-020-03372-8 · Cited by 528
Applications and potential of genome editing in crop improvement
Zhang, Y., Massel, K., Godwin, I. D., & Gao, C. (2018) · Genome Biology · doi:10.1186/s13059-018-1586-y · Cited by 437
Improving cold storage and processing traits in potato through targeted gene knockout
Clasen, B. M., Stoddard, T. J., Luo, S., Demorest, Z. L., Li, J., Cedrone, F., Tibebu, R., Davison, S., Ray, E. E., Daulhac, A., et al. (2016) · Plant Biotechnology Journal · doi:10.1111/pbi.12370 · Cited by 414
Self-citation
Metadata
- Authors
- Haun, W., Coffman, A., Clasen, B. M., Demorest, Z. L., Lowy, A., Ray, E., Retterath, A., Stoddard, T., Juillerat, A., Cedrone, F., Mathis, L., Voytas, D. F., & Zhang, F.
- Venue
- Plant Biotechnology Journal
- Publisher
- Wiley
- Published
- 2014-05-23
- Volume
- 12
- Issue
- 7
- Pages
- 934-940
- ISSN
- 1467-7644 (ISSN-L)
- 1467-7652
- OpenAlex
- W2160906969
- Cited by
- 584
- References
- 20
- FWCI
- 14.24
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Haun, W., Coffman, A., Clasen, B. M., Demorest, Z. L., Lowy, A., Ray, E., Retterath, A., Stoddard, T., Juillerat, A., Cedrone, F., Mathis, L., Voytas, D. F., & Zhang, F. (2014). Improved soybean oil quality by targeted mutagenesis of the fatty acid desaturase 2 gene family. Plant Biotechnology Journal. https://doi.org/10.1111/pbi.12201
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