The DOI matches its Crossref record; 139 of the 139 references it cites were found in a registry or an index.
Bulked sample analysis in genetics, genomics and crop improvement
Open accessAbstract
Summary: Biological assay has been based on analysis of all individuals collected from sample populations. Bulked sample analysis (BSA), which works with selected and pooled individuals, has been extensively used in gene mapping through bulked segregant analysis with biparental populations, mapping by sequencing with major gene mutants and pooled genomewide association study using extreme variants. Compared to conventional entire population analysis, BSA significantly reduces the scale and cost by simplifying the procedure. The bulks can be built by selection of extremes or representative samples from any populations and all types of segregants and variants that represent wide ranges of phenotypic variation for the target trait. Methods and procedures for sampling, bulking and multiplexing are described. The samples can be analysed using individual markers, microarrays and high‐throughput sequencing at all levels of DNA, RNA and protein. The power of BSA is affected by population size, selection of extreme individuals, sequencing strategies, genetic architecture of the trait and marker density. BSA will facilitate plant breeding through development of diagnostic and constitutive markers, agronomic genomics, marker‐assisted selection and selective phenotyping. Applications of BSA in genetics, genomics and crop improvement are discussed with their future perspectives.
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Ma, W., Qiu, Z., Song, J., Li, J., Cheng, Q., Zhai, J., & Ma, C. (2018) · Planta · doi:10.1007/s00425-018-2976-9 · Cited by 341
Enhancing genetic gain in the era of molecular breeding
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Self-citationEnhancing Genetic Gain through Genomic Selection: From Livestock to Plants
Xu, Y., Liu, X., Fu, J., Wang, H., Wang, J., Huang, C., Prasanna, B. M., Olsen, M. S., Wang, G., & Zhang, A. (2020) · Plant Communications · doi:10.1016/j.xplc.2019.100005 · Cited by 281
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Metadata
- Authors
- Zou, C., Wang, P., & Xu, Y.
- Venue
- Plant Biotechnology Journal
- Publisher
- Wiley
- Published
- 2016-04-28
- Volume
- 14
- Issue
- 10
- Pages
- 1941-1955
- ISSN
- 1467-7644 (ISSN-L)
- 1467-7652
- OpenAlex
- W2313533128
- Cited by
- 323
- References
- 139
- FWCI
- 18.35
- Type
- journal article
- Language
- en
- Open access
- gold
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