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Functional and Structural Analysis Reveals Distinct Biological Roles of Plant Synaptotagmins in Response to Environmental Stress
Open accessPublished version of a preprint
Abstract
Endoplasmic reticulum–plasma membrane contact sites (ER–PM CSs) are evolutionarily conserved membrane domains found in all eukaryotes, where the ER closely interfaces with the PM. This short distance is achieved in plants through the action of tether proteins such as synaptotagmins (SYTs). Arabidopsis comprises five SYT members (SYT1 – SYT5), but whether they possess overlapping or distinct biological functions remains elusive. SYT1 , the best‐characterized member, plays an essential role in the resistance to abiotic stress. This study reveals that the functionally redundant SYT1 and SYT3 genes, but not SYT5 , are involved in salt and cold stress resistance. We also show that, unlike SYT5 , SYT1 and SYT3 are not required for Pseudomonas syringae resistance. Since SYT1 and SYT5 interact in vivo via their SMP domains, the distinct functions of these proteins cannot be caused by differences in their localization. Interestingly, structural phylogenetic analysis indicates that the SYT1 and SYT5 clades emerged early in the evolution of land plants. We also show that the SYT1 and SYT5 clades exhibit different structural features in their SMP and Ca 2+ binding of their C2 domains, rationalizing their distinct biological roles.
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Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764
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Metadata
- Authors
- García‐Hernández, S., Rubio, L., Rivera‐Moreno, M., Pérez‐Sancho, J., Morello‐López, J., Esteban del Valle, A., Benítez‐Fuente, F., Beuzón, C. R., Macho, A. P., Ruiz‐López, N., Albert, A., & Botella, M. A.
- Affiliations
- Consejo Superior de Investigaciones Científicas (ES)
- Universidad de Málaga (ES)
- Área de Mejora y Fisiología de Plantas Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Universidad de Málaga‐Consejo Superior de Investigaciones Científicas (IHSM‐UMA‐CSIC) Universidad de Málaga Málaga Spain
- Corresponding author
- Albert, Armando
- Botella, Miguel A.
- ORCID
- Venue
- Plant, Cell & Environment
- Publisher
- Wiley
- Published
- 2024-09-10
- Volume
- 48
- Issue
- 1
- Pages
- 260-271
- ISSN
- 0140-7791 (ISSN-L)
- 1365-3040
- PMC
- PMC11615412
- OpenAlex
- W4402428786
- Cited by
- 14
- References
- 1
- Citations by year
- 2026: 6 · 2025: 7 · 2024: 1
- FWCI
- 3.42
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Plant Cell & Environment (publishedVersion, cc-by-nc-nd)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (acceptedVersion, other-oa)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, other-oa)
- Repositorio Institucional de la Universidad de Málaga (University of Málaga) (submittedVersion, cc-by-nc-nd)
- Funders
- Chinese Academy of Sciences
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Keywords
- synaptotagmin, endoplasmic reticulum-plasma membrane contact sites, Arabidopsis thaliana, synaptotagmin-1, salt tolerance, cold stress resistance, functional redundancy, phylogenetic analysis, plant stress response
- MeSH terms
- Phylogeny, Plant Diseases, Stress, Physiological, Arabidopsis, Arabidopsis Proteins, Pseudomonas syringae, Synaptotagmins, Disease Resistance
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