Strong heterologous electron sink outcompetes alternative electron transport pathways in photosynthesis
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Abstract
Improvement of photosynthesis requires a thorough understanding of electron partitioning under both natural and strong electron sink conditions. We applied a wide array of state-of-the-art biophysical and biochemical techniques to thoroughly investigate the fate of photosynthetic electrons in the engineered cyanobacterium Synechocystis sp. PCC 6803, a blueprint for photosynthetic biotechnology, expressing the heterologous gene for ene-reductase, YqjM. This recombinant enzyme catalyses the reduction of an exogenously added substrate into the desired product by utilising photosynthetically produced NAD(P)H, enabling whole-cell biotransformation. Through coupling the biotransformation reaction with biophysical measurements, we demonstrated that the strong artificial electron sink, outcompetes the natural electron valves, the flavodiiron protein-driven Mehler-like reaction and cyclic electron transport. These results show that ferredoxin-NAD(P)H-oxidoreductase is the preferred route for delivering photosynthetic electrons from reduced ferredoxin and the cellular NADPH/NADP+ ratio as a key factor in orchestrating photosynthetic electron flux. These insights are crucial for understanding molecular mechanisms of photosynthetic electron transport and harnessing photosynthesis for sustainable bioproduction by engineering the cellular source/sink balance. Furthermore, we conclude that identifying the bioenergetic bottleneck of a heterologous electron sink is a crucial prerequisite for targeted engineering of photosynthetic biotransformation platforms.
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Engineered pathways demanding substantial reducing power may compete with native metabolic processes for cofactors, potentially constraining overall pathway performance.
“This dynamic means that engineered pathways demanding substantial reducing power may compete with native metabolic processes for these cofactors, thereby potentially constraining overall pathway performance [172].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [172]
In cyanobacteria, intracellular levels of ATP and NADPH are closely tied to photosynthetic electron transport, and variations in light intensity, carbon sources, or environmental conditions can alter the cell's redox state.
“In cyanobacteria, the intracellular levels of ATP and NADPH are closely tied to PET. Consequently, variations in light intensity, carbon sources, or environmental conditions can alter the cell’s redox state.”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [172]
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Effects of Photosynthesis Inhibitors on H2 Production in Microalgae and Cyanobacteria
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- Authors
- Hubáček, M., Wey, L. T., Kourist, R., Malihan‐Yap, L., Nikkanen, L., & Allahverdiyeva, Y.
- Affiliations
- University of Turku (FI)
- Graz University of Technology (AT)
- Corresponding author
- Allahverdiyeva, Yagut
- ORCID
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2024-07-15
- Volume
- 119
- Issue
- 5
- Pages
- 2500-2513
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W4400648575
- Cited by
- 15
- References
- 79
- Citations by year
- 2026: 7 · 2025: 7 · 2024: 1
- FWCI
- 2.03
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- The Plant Journal (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed (publishedVersion)
- UTUPub (University of Turku) (submittedVersion)
- UTUPub (University of Turku) (submittedVersion, cc-by)
- Funders
- European Commission (899576)
- Academy of Finland (315119, 322754)
- Novo Nordisk Fonden (NNF20OC0064371)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Keywords
- photosynthetic electron transport, Synechocystis sp. PCC 6803, whole-cell biocatalysis, Mehler reaction, cyclic electron flow, electron partitioning, NADPH/NADP+ ratio, flavodiiron proteins
- MeSH terms
- Bacterial Proteins, Electron Transport, Electrons, Ferredoxin-NADP Reductase, Ferredoxins, NADP, Photosynthesis, Synechocystis
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