The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Supernatants from stored red blood cell (RBC) units, but not RBC-derived microvesicles, suppress monocyte function in vitro.
Abstract
BACKGROUND: We have previously shown that critically ill children transfused with red blood cells (RBCs) of longer storage durations have more suppressed monocyte function after transfusion compared to children transfused with fresher RBCs and that older stored RBCs directly suppress monocyte function in vitro, through unknown mechanisms. We hypothesized that RBC‐derived microvesicles (MVs) were responsible for monocyte suppression. STUDY DESIGN AND METHODS: To determine the role of stored RBC unit–derived MVs, we cocultured monocytes with supernatants, isolated MVs, or supernatants that had been depleted of MVs from prestorage leukoreduced RBCs that had been stored for either 7 or 30 days. Isolated MVs were characterized by electron microscopy and flow cytometry. Monocyte function after coculture experiments was measured by cytokine production after stimulation with lipopolysaccharide (LPS). RESULTS: Monocyte function was suppressed after exposure to supernatants from 30‐day RBC units compared to monocytes cultured in medium alone (LPS‐induced tumor necrosis factor‐α production, 17,611 ± 3,426 vs. 37,486 ± 5,598 pg/mL; p = 0.02). Monocyte function was not suppressed after exposure to MV fractions. RBC supernatants that had been depleted of MVs remained immunosuppressive. Treating RBC supernatants with heat followed by RNase (to degrade protein‐bound RNA) prevented RBC supernatant–induced monocyte suppression. CONCLUSION: Our findings implicate soluble mediators of stored RBC‐induced monocyte suppression outside of MV fractions and suggest that extracellular protein‐bound RNAs (such as microRNA) may play a role in transfusion‐related immunomodulation.
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In the citing paper
“which indicates that Ago-miRNAs derived from RBC, as a major protein–miRNA complex form, are likely transported to other recipient cells to exert their function.”
From Red Blood Cells as Potential Repositories of MicroRNAs in the Circulatory System (2020) · cites this paper as Muszynski et al., 2015
In the citing paper
“Another study evaluated protein-bound miRNAs in RBC supernatants and showed that these induce monocyte suppression”
From Red Blood Cells as Potential Repositories of MicroRNAs in the Circulatory System (2020) · cites this paper as Muszynski et al., 2015
Citations
Cites
Arroyo, J. D., Chevillet, J. R., Kroh, E. M., Ruf, I. K., Pritchard, C. C., Gibson, D. F., Mitchell, P. S., Bennett, C. F., Pogosova-Agadjanyan, E. L., Stirewalt, D. L., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1019055108 · Cited by 3,294
Exosomes: Extracellular organelles important in intercellular communication
Mathivanan, S., Ji, H., & Simpson, R. J. (2010) · Journal of Proteomics · doi:10.1016/j.jprot.2010.06.006 · Cited by 2,537
Duration of Red-Cell Storage and Complications after Cardiac Surgery
Koch, C. G., Li, L., Sessler, D. I., Figueroa, P., Hoeltge, G. A., Mihaljevic, T., & Blackstone, E. H. (2008) · New England Journal of Medicine · doi:10.1056/nejmoa070403 · Cited by 1,363
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Opelz, G., SENGAR, D. P. S., Mickey, M. R., & Terasaki, P. I. (1973) · PubMed · Cited by 845
Blood Transfusion, Independent of Shock Severity, Is Associated with Worse Outcome in Trauma
Malone, D. L., Dunne, J., Tracy, J. K., Putnam, A. T., Scalea, T. M., & Napolitano, L. M. (2003) · The Journal of Trauma: Injury, Infection, and Critical Care · doi:10.1097/01.ta.0000060261.10597.5c · Cited by 616
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Persisting low monocyte human leukocyte antigen-DR expression predicts mortality in septic shock
Monneret, G., Lepape, A., Voirin, N., Bohé, J., Venet, F., Debard, A.-L., Thizy, H., Bienvenu, J., Gueyffier, F., & Vanhems, P. (2006) · Intensive Care Medicine · doi:10.1007/s00134-006-0204-8 · Cited by 545
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Monocyte deactivation-rationale for a new therapeutic strategy in sepsis
Volk, H. D., Reinke, P., Krausch, D., Zuckermann, H., Asadullah, K., Müller, J. M., Döcke, W. D., & Kox, W. J. (1996) · Intensive Care Medicine · doi:10.1007/bf01743727 · Cited by 361
Research works printed but not linked: 1
- Ploder M, 2006, Lipopolysaccharide‐induced tumor necrosis factor alpha production and not monocyte human leukocyte antigen‐DR expression is correlated with survival in septic trauma patients, Shock, 25, 129
Cited by
Red Blood Cells as Potential Repositories of MicroRNAs in the Circulatory System
Sun, L., Yu, Y., Niu, B., & Wang, D. (2020) · Frontiers in Genetics · doi:10.3389/fgene.2020.00442 · Cited by 83
Huang, H., Zhu, J., Fan, L., Lin, Q., Fu, D., Wei, B., & Wei, S. (2019) · BioMed Research International · doi:10.1155/2019/2045915 · Cited by 33
Metadata
- Authors
- Muszynski, J. A., Bale, J., Nateri, J., Nicol, K., Wang, Y., Wright, V., Marsh, C. B., Gavrilin, M. A., Sarkar, A., Wewers, M. D., & Hall, M. W.
- Affiliations
- Division of Critical Care Medicine
- The Research Institute
- Department of Pathology Nationwide Children's Hospital
- Division of Pulmonary and Critical Care Department of Internal Medicine The Ohio State University Columbus Ohio
- Venue
- Transfusion
- Publisher
- Wiley
- Published
- 2015-03-27
- Volume
- 55
- Issue
- 8
- Pages
- 1937-1945
- ISSN
- 0041-1132
- 1537-2995
- Cited by
- 39
- References
- 46
- Language
- en
- Funders
- National Institutes of Health (K12HD043372)
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- The paper
- Muszynski, J. A., Bale, J., Nateri, J., Nicol, K., Wang, Y., Wright, V., Marsh, C. B., Gavrilin, M. A., Sarkar, A., Wewers, M. D., & Hall, M. W. (2015). Supernatants from stored red blood cell (RBC) units, but not RBC-derived microvesicles, suppress monocyte function in vitro. Transfusion. https://doi.org/10.1111/trf.13084
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