Motor units in cat soleus muscle: physiological, histochemical and morphological characteristics

Abstract

1. Physiological properties of motor units in the soleus muscle were studied in anaesthetized cats using intracellular stimulation of motoneurones to ensure functional isolation of single units. The muscle fibres belonging to 6 units were identified by glycogen depletion following prolonged stimulation, permitting analysis of their histochemical profiles and anatomical organization. 2. The studied units in soleus were all classed as type S and were extremely resistant to fatigue during prolonged stimulation. Twitch contraction times ranged from 64 to 131 msec (mean 97·1 msec) and tetanic tensions ranged from 3·5 to 36 g (mean 10·5 g). Most units exhibited depression of twitch tension in the wake of a short high‐frequency tetanus and few of the units showed any significant degree of post‐tetanic twitch potentiation. 3. Muscle fibres belonging to single soleus motor units were found to be scattered through territorial volumes occupying a large fraction of the total muscle volume. The available data suggest that different soleus motoneurones may innervate from less than 50 to more than 400 muscle fibres, with an average innervation ratio between 140 and 190 muscle fibres per unit. 4. The results were compared with observations on type S motor units in the synergist gastrocnemius, obtained under similar conditions. The evidence suggests that soleus units are not equivalent to the type S units of the mixed gastrocnemius but rather constitute a unique population.