The formation of synapses in striated muscle during development

Abstract

1. A study has been made of the formation of synapses in developing striated muscles which receive either a focal (the rat hemidiaphragm) or a distributed (the avian anterior latissimus dorsi) innervation using histological, ultrastructural and electrophysiological techniques. 2. In the developing diaphragm only a single synaptic contact was initially established at random along the length of the short (300 μm) myotubes by a single axon; in the developing ALD more than one synaptic contact could be established initially along the length of the long (2500 μm) myotubes by axons, but the distance between these was never less than 170 μm. 3. Each synapse established by the initial axonal contact in either the diaphragm or the ALD subsequently received a multiple innervation from further exploring axons in the muscles, and all such additional innervation of muscle cells was constrained to the sites of the initial synaptic contacts; this multiple innervation of synaptic sites was lost in the subsequent 4 weeks. 4. It is suggested that the axon forming the initial synaptic contact on myotubes induces a property over an adjacent length of myotube which makes its membrane refractory to synapse formation over this length; this characteristic length is longer for axons forming a focal innervation than it is for those forming distributed innervation.