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Cited by 21Cardiovascular and exercise physiologyMuscle metabolism and nutritionMitochondrial Function and Pathology

An acute decrease in TCA cycle intermediates does not affect aerobic energy delivery in contracting rat skeletal muscle

Dawson, Kristen D., Baker, David J., Greenhaff, Paul L., Gibala, Martin J.·2005·The Journal of Physiology
DOI 10.1113/jphysiol.2004.079939
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Abstract

We tested the hypothesis that an acute decrease in muscle TCA cycle intermediates during contraction would compromise aerobic energy delivery. Male Wistar rats were anaesthetized and the gastrocnemius-plantaris-soleus (GPS) muscle complex from one leg was isolated and perfused with a red cell medium containing either saline (Con) or cycloserine (Cyclo; 0.05 mg g-1), an inhibitor of alanine aminotransferase (AAT). After 1 h of perfusion, the GPS muscle was either snap frozen (Con-Rest, n=11; Cyclo-Rest, n=9) or stimulated to contract for 10 min (1 Hz, 0.3 ms, 2 V) with blood flow fixed at 30 ml min-1 (100 g)-1 and then snap frozen (Con-Stim, n=10; Cyclo-Stim, n=10). Maximal AAT activity was>80% lower (P<0.001) in both Cyclo-treated groups (Rest: 0.61+/-0.02; Stim: 0.63+/-0.01 mmol (kg wet wt)-1 min-1; mean+/-s.e.m.) compared to Con (Rest: 3.56+/-0.16; Stim: 3.92+/-0.29). The sum of five measured TCAI (SigmaTCAI) was reduced by 23% in Cyclo-Rest versus Con-Rest but this was not different (P=0.08). However, after 10 min of contraction, the SigmaTCAI was 25% lower (P=0.006) in Cyclo-Stim compared to Con-Stim (1.88+/-0.15 versus 2.48+/-0.11 mmol (kg dry wt)-1). Despite the acute decrease in TCAI after Cyclo treatment, the contraction-induced changes in markers of non-oxidative energy provision (phosphocreatine, ATP and lactate) and the decline in tension after 10 min of stimulation were similar compared to Con. These data do not support the hypothesis that the total muscle concentration of TCAI is causally linked to the rate of mitochondrial respiration during contraction.

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  • Data from animal studies appear to support the notion that aerobic energy delivery is unaffected by TCA cycle reductions.

    “Data from animal studies appear to support the notion that aerobic energy delivery is unaffected by TCA cycle reductions [183].”

    From Regulation of Energy Substrate Metabolism in Endurance Exercise (2021) · cites this paper as [183]

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Authors
Dawson, K. D., Baker, D. J., Greenhaff, P. L., & Gibala, M. J.
Affiliations
  • McMaster University (CA)
  • University of Nottingham (GB)
ORCID
Venue
The Journal of Physiology
Publisher
Wiley
Published
2005-06
Volume
565
Issue
2
Pages
637-643
ISSN
  • 0022-3751 (ISSN-L)
  • 1469-7793
DOI
10.1113/jphysiol.2004.079939
OpenAlex
W1561775546
Cited by
21
References
30
Citations by year
2024: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2014: 3
FWCI
6.48
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 12 d)
Locations
  • The Journal of Physiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Natural Sciences and Engineering Research Council of Canada
Topics
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
Keywords
TCA cycle intermediates, skeletal muscle contraction, cycloserine, mitochondrial respiration, phosphocreatine, ATP, lactate
MeSH terms
Alanine Transaminase, Amino Acids, Animals, Citric Acid Cycle, Energy Metabolism, Physical Exertion, Glycogen, Male, Mitochondria, Muscle Contraction, Rats, Wistar, Muscle, Skeletal, Rats

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