The DOI matches its Crossref record.
Scanning light-sheet microscopy in the whole mouse brain with HiLo background rejection
Open accessAbstract
It is well known that light-sheet illumination can enable optically sectioned wide-field imaging of macroscopic samples. However, the optical sectioning capacity of a light-sheet macroscope is undermined by sample-induced scattering or aberrations that broaden the thickness of the sheet illumination. We present a technique to enhance the optical sectioning capacity of a scanning light-sheet microscope by out-of-focus background rejection. The technique, called HiLo microscopy, makes use of two images sequentially acquired with uniform and structured sheet illumination. An optically sectioned image is then synthesized by fusing high and low spatial frequency information from both images. The benefits of combining light-sheet macroscopy and HiLo background rejection are demonstrated in optically cleared whole mouse brain samples, using both green fluorescent protein (GFP)-fluorescence and dark-field scattered light contrast.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
On the use of deep learning for computational imaging
Barbastathis, G., Ozcan, A., & Situ, G. (2019) · Optica · doi:10.1364/optica.6.000921 · Cited by 869
Light Sheet Fluorescence Microscopy: A Review
Santi, P. A. (2011) · Journal of Histochemistry & Cytochemistry · doi:10.1369/0022155410394857 · Cited by 431
Optical sectioning microscopy with planar or structured illumination
Mertz, J. (2011) · Nature Methods · doi:10.1038/nmeth.1709 · Cited by 347
Self-citationLight-sheet microscopy: a tutorial
Olarte, O. E., Andilla, J., Gualda, E. J., & Loza-Alvarez, P. (2018) · Advances in Optics and Photonics · doi:10.1364/aop.10.000111 · Cited by 298
Single Cell Optical Imaging and Spectroscopy
Stender, A. S., Marchuk, K., Liu, C., Sander, S., Meyer, M. W., Smith, E. A., Neupane, B., Wang, G., Li, J., Cheng, J.-X., et al. (2013) · Chemical Reviews · doi:10.1021/cr300336e · Cited by 292
Metadata
- Authors
- Mertz, J., & Kim, J.
- Venue
- Journal of Biomedical Optics
- Publisher
- SPIE-Intl Soc Optical Eng
- Published
- 2010-01-01
- Volume
- 15
- Issue
- 01
- Pages
- 1
- ISSN
- 1083-3668 (ISSN-L)
- OpenAlex
- W2098681947
- Cited by
- 183
- References
- 0
- FWCI
- 6.74
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Scanning light-sheet microscopy in the whole mouse brain with HiLo background rejection. https://app.cochat.ai/papers/10.1117/1.3324890
- The paper
- Mertz, J., & Kim, J. (2010). Scanning light-sheet microscopy in the whole mouse brain with HiLo background rejection. Journal of Biomedical Optics. https://doi.org/10.1117/1.3324890
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
