A lineage-specific Exo70 is required for receptor kinase–mediated immunity in barley
Open accessPublished version of a preprint
Abstract
In the evolution of land plants, the plant immune system has experienced expansion in immune receptor and signaling pathways. Lineage-specific expansions have been observed in diverse gene families that are potentially involved in immunity but lack causal association. Here, we show that Rps8 -mediated resistance in barley to the pathogen Puccinia striiformis f. sp. tritici (wheat stripe rust) is conferred by a genetic module: Pur1 and Exo70FX12 , which are together necessary and sufficient. Pur1 encodes a leucine-rich repeat receptor kinase and is the ortholog of rice Xa21 , and Exo70FX12 belongs to the Poales-specific Exo70FX clade. The Exo70FX clade emerged after the divergence of the Bromeliaceae and Poaceae and comprises from 2 to 75 members in sequenced grasses. These results demonstrate the requirement of a lineage-specific Exo70FX12 in Pur1-mediated immunity and suggest that the Exo70FX clade may have evolved a specialized role in receptor kinase signaling.
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Cites
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- Authors
- Holden, S., Bergum, M., Green, P., Bettgenhaeuser, J., Hernández-Pinzón, I., Thind, A., Clare, S., Russell, J. M., Hubbard, A., Taylor, J., Smoker, M., Gardiner, M., Civolani, L., Cosenza, F., Rosignoli, S., Strugala, R., Molnár, I., Šimková, H., Doležel, J., Schaffrath, U., Barrett, M., Salvi, S., & Moscou, M. J.
- Affiliations
- University of East Anglia (GB)
- The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich NR4 7UH, UK.
- National Institute of Agricultural Botany (GB)
- University of Bologna (IT)
- RWTH Aachen University (DE)
- Centre of the Region Haná for Biotechnical and Agricultural Research (CZ)
- Australian Tropical Herbarium (AU)
- Corresponding author
- Moscou, Matthew J.
- ORCID
- Holden, Samuel
- Bergum, Molly
- Green, Phon
- Bettgenhaeuser, Jan
- Hernández-Pinzón, Inmaculada
- Thind, Anupriya
- Clare, Shaun
- Russell, James M.
- Hubbard, Amelia
- Taylor, Jodi
- Smoker, Matthew
- Gardiner, Matthew
- Civolani, Laura
- Cosenza, Francesco
- Rosignoli, Serena
- Molnár, István
- Šimková, Hana
- Doležel, Jaroslav
- Schaffrath, Ulrich
- Barrett, Matthew
- Salvi, Silvio
- Moscou, Matthew J.
- Venue
- Science Advances
- Published
- 2022-07-08
- Volume
- 8
- Issue
- 27
- Pages
- eabn7258-eabn7258
- ISSN
- 2375-2548 (ISSN-L)
- PMC
- PMC9258809
- OpenAlex
- W4283826677
- Cited by
- 29
- References
- 3
- Citations by year
- 2026: 5 · 2025: 6 · 2024: 9 · 2023: 7 · 2022: 2
- FWCI
- 3.30
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Science Advances (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Archivio istituzionale della ricerca (Alma Mater Studiorum Università di Bologna) (submittedVersion, cc-by)
- RWTH Publications (RWTH Aachen)
- Funders
- U.S. Department of Agriculture
- UK Research and Innovation (BB/M011216/1)
- Agricultural Research Service
- Biotechnology and Biological Sciences Research Council (M011216, BB/P012574/1, BB/P012574, BB/M011216/1, BBS/E/J/000PR9795)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Wheat and Barley Genetics and Pathology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Disease Resistance and Genetics
- Keywords
- ribosomal protein S8, barley, Puccinia striiformis f. sp. tritici, wheat stripe rust, receptor kinase signaling, leucine-rich repeat receptor-like kinases, plant immunity, lineage-specific gene expansion, Poaceae, Poales, Xa21, disease resistance
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