Cited by 95Wheat and Barley Genetics and PathologyChromosomal and Genetic VariationsGenomics and Phylogenetic Studies

A Genome Assembly of the Barley ‘Transformation Reference’ Cultivar Golden Promise

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Schreiber, Miriam, Mascher, Martin, Wright, Jonathan, Padmarasu, Sudharasan, Himmelbach, Axel, Heavens, Darren, Milne, Linda, Clavijo, Bernardo J +2 more·2020·G3 Genes|Genomes|Genetics

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Abstract

Barley (Hordeum vulgare) is one of the most important crops worldwide and is also considered a research model for the large-genome small grain temperate cereals. Despite genomic resources improving all the time, they are limited for the cv. Golden Promise, the most efficient genotype for genetic transformation. We have developed a barley cv. Golden Promise reference assembly integrating Illumina paired-end reads, long mate-pair reads, Dovetail Chicago in vitro proximity ligation libraries and chromosome conformation capture sequencing (Hi-C) libraries into a contiguous reference assembly. The assembled genome of 7 chromosomes and 4.13Gb in size, has a super-scaffold N50 after Chicago libraries of 4.14Mb and contains only 2.2% gaps. Using BUSCO (benchmarking universal single copy orthologous genes) as evaluation the genome assembly contains 95.2% of complete and single copy genes from the plant database. A high-quality Golden Promise reference assembly will be useful and utilized by the whole barley research community but will prove particularly useful for CRISPR-Cas9 experiments.

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Authors
Schreiber, M., Mascher, M., Wright, J., Padmarasu, S., Himmelbach, A., Heavens, D., Milne, L., Clavijo, B. J., Stein, N., & Waugh, R.
Affiliations
  • James Hutton Institute (GB)
  • German Centre for Integrative Biodiversity Research (DE)
  • Norwich Research Park (GB)
  • Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Germany
  • Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) (DE)
Corresponding author
Waugh, Robbie
Venue
G3 Genes|Genomes|Genetics
Published
2020-06-01
Volume
10
Issue
6
Pages
1823-1827
ISSN
2160-1836 (ISSN-L)
OpenAlex
W3015028662
Cited by
94
References
30
Citations by year
2026: 7 · 2025: 10 · 2024: 17 · 2023: 15 · 2022: 18 · 2021: 21 · 2020: 6
FWCI
9.69
Type
journal article
Language
en
Open access
Locations
  • G3 Genes Genomes Genetics (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Adelaide Research & Scholarship (AR&S) (University of Adelaide) (submittedVersion, cc-by)
  • Discovery Research Portal (University of Dundee) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Rural and Environment Science and Analytical Services Division
  • Scottish Government
  • Bundesministerium für Bildung und Forschung (031B0190A)
  • Horizon 2020 Framework Programme
  • Biotechnology and Biological Sciences Research Council (BBS/E/T/000PR9817, BB/I00663X/1, BBS/E/T/000PR5885, BBS/E/T/000PR9818, BBS/E/T/000PR9819, BBS/E/T/000PR6193)
  • H2020 European Research Council (ERC Shuffle, Project ID: 66918, H2020)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Wheat and Barley Genetics and Pathology
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
Keywords
barley, Golden Promise, genome assembly, reference genome, genetic transformation, CRISPR/Cas9, chromosome conformation capture, Hi-C, long-read sequencing
MeSH terms
Hordeum, Genotype, Genome, Genomics, High-Throughput Nucleotide Sequencing

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