A Genome Assembly of the Barley ‘Transformation Reference’ Cultivar Golden Promise
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Abstract
Barley (Hordeum vulgare) is one of the most important crops worldwide and is also considered a research model for the large-genome small grain temperate cereals. Despite genomic resources improving all the time, they are limited for the cv. Golden Promise, the most efficient genotype for genetic transformation. We have developed a barley cv. Golden Promise reference assembly integrating Illumina paired-end reads, long mate-pair reads, Dovetail Chicago in vitro proximity ligation libraries and chromosome conformation capture sequencing (Hi-C) libraries into a contiguous reference assembly. The assembled genome of 7 chromosomes and 4.13Gb in size, has a super-scaffold N50 after Chicago libraries of 4.14Mb and contains only 2.2% gaps. Using BUSCO (benchmarking universal single copy orthologous genes) as evaluation the genome assembly contains 95.2% of complete and single copy genes from the plant database. A high-quality Golden Promise reference assembly will be useful and utilized by the whole barley research community but will prove particularly useful for CRISPR-Cas9 experiments.
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A reference genome assembly for 'Golden Promise' is now available.
“A reference genome assembly for this genotype is now available (Schreiber et al., 2020).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Schreiber et al., 2020
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Local adjustment of exodermis to nutrient deficiency in crop species
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- Authors
- Schreiber, M., Mascher, M., Wright, J., Padmarasu, S., Himmelbach, A., Heavens, D., Milne, L., Clavijo, B. J., Stein, N., & Waugh, R.
- Affiliations
- James Hutton Institute (GB)
- German Centre for Integrative Biodiversity Research (DE)
- Norwich Research Park (GB)
- Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Germany
- Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) (DE)
- Corresponding author
- Waugh, Robbie
- ORCID
- Venue
- G3 Genes|Genomes|Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 2020-06-01
- Volume
- 10
- Issue
- 6
- Pages
- 1823-1827
- ISSN
- 2160-1836 (ISSN-L)
- PMC
- PMC7263683
- OpenAlex
- W3015028662
- Cited by
- 94
- References
- 30
- Citations by year
- 2026: 7 · 2025: 10 · 2024: 17 · 2023: 15 · 2022: 18 · 2021: 21 · 2020: 6
- FWCI
- 9.69
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- G3 Genes Genomes Genetics (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Adelaide Research & Scholarship (AR&S) (University of Adelaide) (submittedVersion, cc-by)
- Discovery Research Portal (University of Dundee) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Rural and Environment Science and Analytical Services Division
- Scottish Government
- Bundesministerium für Bildung und Forschung (031B0190A)
- Horizon 2020 Framework Programme
- Biotechnology and Biological Sciences Research Council (BBS/E/T/000PR9817, BB/I00663X/1, BBS/E/T/000PR5885, BBS/E/T/000PR9818, BBS/E/T/000PR9819, BBS/E/T/000PR6193)
- H2020 European Research Council (ERC Shuffle, Project ID: 66918, H2020)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Wheat and Barley Genetics and Pathology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Keywords
- barley, Golden Promise, genome assembly, reference genome, genetic transformation, CRISPR/Cas9, chromosome conformation capture, Hi-C, long-read sequencing
- MeSH terms
- Hordeum, Genotype, Genome, Genomics, High-Throughput Nucleotide Sequencing
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